Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects Journal Article


Authors: Grüner, F.; Blumendorf, F.; Schmutzler, O.; Staufer, T.; Bradbury, M.; Wiesner, U.; Rosentreter, T.; Loers, G.; Lutz, D.; Richter, B.; Fischer, M.; Schulz, F.; Steiner, S.; Warmer, M.; Burkhardt, A.; Meents, A.; Kupinski, M.; Hoeschen, C.
Article Title: Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
Abstract: Accurate in vivo localisation of minimal amounts of functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal and spatial resolution, large depth penetration, and arbitrarily long serial measurements. X-ray fluorescence imaging could offer such capabilities; however, its utilisation for human-sized scales is hampered by a high intrinsic background level. Here we measure and model this anisotropic background and present a spatial filtering scheme for background reduction enabling the localisation of nanoparticle-amounts as reported from small-animal tumour models. As a basic application study towards precision pharmacokinetics, we demonstrate specific localisation to sites of disease by adapting gold-nanoparticles with small targeting ligands in murine spinal cord injury models, at record sensitivity levels using sub-mm resolution. Both studies contribute to the future use of molecularly-targeted gold-nanoparticles as next-generation clinical diagnostic and pharmacokinetic tools. © 2018, The Author(s).
Journal Title: Scientific Reports
Volume: 8
ISSN: 2045-2322
Publisher: Nature Publishing Group  
Date Published: 2018-11-08
Start Page: 16561
Language: English
DOI: 10.1038/s41598-018-34925-3
PUBMED: 30410002
PROVIDER: scopus
PMCID: PMC6224495
DOI/URL:
Notes: Article -- Export Date: 3 December 2018 -- Source: Scopus
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