Jiedu Sangen Decoction inhibits migration and invasion of colon cancer SW480 cells via suppressing epithelial mesenchymal transition Journal Article


Authors: Zhang, K.; Peng, T.; Yan, Q.; Sun, L.; Miao, H.; Yuan, L.; Hong, Y.; Zhou, M.; Guo, K.; Zhang, L.; Fang, J.; Shen, M.; Ruan, S.
Article Title: Jiedu Sangen Decoction inhibits migration and invasion of colon cancer SW480 cells via suppressing epithelial mesenchymal transition
Abstract: Jiedu Sangen Decoction (JSD), a traditional Chinese medicine (TCM) formula, has been widely used in China to treat gastrointestinal cancer, especially as an adjuvant therapy in colorectal cancer (CRC) patients. This study aimed to evaluate the efficacy of JSD and Jiedu Sangen aqueous extract (JSAE) in colon cancer cells and explored the underlining mechanisms by cytotoxicity assay, scratch assay, transwell migration assay, matrigel invasion assay, confocal laser scanning microscopy, and western blot analysis. We demonstrated that JSAE inhibited the growth of colon cancer SW480 cells in a dose-dependent manner and JSAE repressed cancer cell migration and invasion. Furthermore, epithelial mesenchymal transition (EMT) was reversed by JSAE via enhancing E-cadherin expression and attenuating protein levels of EMT promoting factors such as N-cadherin, Slug, and ZEB1. These findings provided the first experimental evidence confirming the efficacy of JSAE in repressing invasion and metastasis of CRC and paving a way for the broader use of JSD in clinic. © 2018 Kai Zhang et al.
Journal Title: Evidence-Based Complementary and Alternative Medicine
Volume: 2018
ISSN: 1741-427X
Publisher: Hindawi Publishing Corporation  
Date Published: 2018-09-25
Start Page: 1495768
Language: English
DOI: 10.1155/2018/1495768
PROVIDER: scopus
PMCID: PMC6176311
PUBMED: 30356400
DOI/URL:
Notes: Article -- Export Date: 3 December 2018 -- Source: Scopus
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  1. Kai Zhang
    1 Zhang