Selective cytotoxicity of Y-90 labeled T101 monoclonal antibody on human malignant T-cell lines Journal Article

  


Authors: Naruki, Y.; Maloney, P. J.; Reynolds, J. C.; Larson, S. M.; Carrasquillo, J. A.
Article Title: Selective cytotoxicity of Y-90 labeled T101 monoclonal antibody on human malignant T-cell lines
Abstract: Yttrium-90 radiolabeled antibodies are currently being evaluated as radioimmunotherapeutic reagents. In this study we evaluated whether Y-90 labeled T101, a murine IgG2a monoclonal antibody (MoAb) that recognizes CD5 could selectively induce cytotoxic damage to cells in culture. Two CD5 positive cell lines, HUT102 and CCRF-CEM cells and PGT a CD5 negative cell line was treated with 0.005 to 5 mu Ci of Y-90 T101. After 48 and 72 hrs incubation, cytotoxicity was measured using thymidine incorporation as a marker. In addition to treatment with Y-90 labeled T101, control studies included Y-90 DTPA, Y-90 96.5 (non-specific MoAb), and excess unlabeled T101 prior to Y-90 T101. Selective cytotoxicity was observed with the Y-90 T101.
Keywords: protein; lymphoma; toxicity; radioimmunotherapy; lymphocytes; radioimmunodetection; in-111; radionuclides
Journal Title: Antibody, Immunoconjugates, and Radiopharmaceuticals
Volume: 8
Issue: 2
ISSN: 0892-7049
Publisher: Mary Ann Liebert, Inc.  
Date Published: 1995-01-01
Start Page: 81
End Page: 92
Language: English
ACCESSION: WOS:A1995RL32700001
PROVIDER: wos
Notes: The PDF lists the publication date as Summer -- Article -- Source: Wos