An optical nanoreporter of endolysosomal lipid accumulation reveals enduring effects of diet on hepatic macrophages in vivo Journal Article


Authors: Galassi, T. V.; Jena, P. V.; Shah, J.; Ao, G.; Molitor, E.; Bram, Y.; Frankel, A.; Park, J.; Jessurun, J.; Ory, D. S.; Haimovitz-Friedman, A.; Roxbury, D.; Mittal, J.; Zheng, M.; Schwartz, R. E.; Heller, D. A.
Article Title: An optical nanoreporter of endolysosomal lipid accumulation reveals enduring effects of diet on hepatic macrophages in vivo
Abstract: The abnormal accumulation of lipids within the endolysosomal lumen occurs in many conditions, including lysosomal storage disorders, atherosclerosis, nonalcoholic fatty liver disease (NAFLD), and drug-induced phospholipidosis. Current methods cannot monitor endolysosomal lipid content in vivo, hindering preclinical drug development and research into the mechanisms linking endolysosomal lipid accumulation to disease progression. We developed a single-walled carbon nanotube–based optical reporter that noninvasively measures endolysosomal lipid accumulation via bandgap modulation of its intrinsic near-infrared emission. The reporter detected lipid accumulation in Niemann-Pick disease, atherosclerosis, and NAFLD models in vivo. By applying the reporter to the study of NAFLD, we found that elevated lipid quantities in hepatic macrophages caused by a high-fat diet persist long after reverting to a normal diet. The reporter dynamically monitored endolysosomal lipid accumulation in vivo over time scales ranging from minutes to weeks, indicating its potential to accelerate preclinical research and drug development processes. Copyright © 2018 The Authors, some rights reserved.
Journal Title: Science Translational Medicine
Volume: 10
Issue: 461
ISSN: 1946-6234
Publisher: American Association for the Advancement of Science  
Date Published: 2018-10-03
Start Page: eaar2680
Language: English
DOI: 10.1126/scitranslmed.aar2680
PUBMED: 30282694
PROVIDER: scopus
PMCID: PMC6543545
DOI/URL:
Notes: Article -- Export Date: 1 November 2018 -- Source: Scopus
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MSK Authors
  1. Daniel Alan Heller
    102 Heller
  2. Prakrit Vaibhav Jena
    27 Jena
  3. Janki Kalpesh Shah
    23 Shah
  4. Thomas Vito Galassi
    13 Galassi