A strategy for a convergent synthesis of N-linked glycopeptides on a solid support Journal Article
| Authors: | Roberge, J. Y.; Beebe, X.; Danishefsky, S. J. |
| Article Title: | A strategy for a convergent synthesis of N-linked glycopeptides on a solid support |
| Abstract: | Oligosaccharides and glycopeptides are of considerable importance in molecular biology and pharmacology. However, their synthesis is complicated by the large number of different linking sites between each saccharide unit, the need for stereochemical control, the chemical sensitivity of the glycopeptide bonds, and the need to harmonize diverse protecting groups. Here, an efficient solid-phase synthesis of three N-linked glycopeptides based on glycal assembly is presented. The peptide domain can be extended while the ensemble remains bound to the polymer. The glycopeptides synthesized here are among the largest N-linked glycopeptides ever accessed by either solution- or solid-phase synthesis. |
| Keywords: | amino acid sequence; molecular sequence data; protein purification; peptides; magnetic resonance spectroscopy; nuclear magnetic resonance; polymer; carbohydrate sequence; glycopeptide; glycopeptides; stereochemistry; oligosaccharide; carbohydrate conformation; oligosaccharides; carbohydrate synthesis; priority journal; article; column chromatography; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Science |
| Volume: | 269 |
| Issue: | 5221 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 1995-07-14 |
| Start Page: | 202 |
| End Page: | 204 |
| Language: | English |
| DOI: | 10.1126/science.7618080 |
| PUBMED: | 7618080 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
