Comparative genomic analysis of tumors: Detection of DNA losses and amplification Journal Article
| Authors: | Lisitsyn, N. A.; Lisitsina, N. M.; Dalbagni, G.; Barker, P.; Sanchez, C. A.; Gnarra, J.; Linehan, W. M.; Reid, B. J.; Wigler, M. H. |
| Article Title: | Comparative genomic analysis of tumors: Detection of DNA losses and amplification |
| Abstract: | We demonstrate the use of representational difference analysis for cloning probes that detect DNA loss and amplification in tumors. Using DNA isolated from human tumor cell lines to drive hybridization against matched normal DNA, we were able to identify six genomic regions that are homozygously deleted in cultured cancer cells. When this method was applied in the reverse way, using normal DNA to drive hybridization against tumor cell DNA, we readily isolated probes detecting amplification. Representational difference analysis was also performed on DNAs derived from tumor biopsies, and we thereby discovered a probe detecting very frequent homozygous loss in colon cancer cell lines and located on chromosome 3p. |
| Keywords: | controlled study; human cell; comparative study; polymerase chain reaction; neoplasms; animal; in situ hybridization, fluorescence; melanoma; gene amplification; analytic method; cell line; colonic neoplasms; tumor biopsy; cancer cell culture; tumor cells, cultured; breast neoplasms; kidney neoplasms; oncogenes; colorectal neoplasms; cancer genetics; oncogene; dna; molecular sequence data; carcinoma, renal cell; glioblastoma; dna, neoplasm; gene loss; base sequence; bladder neoplasms; rodentia; dna primers; chromosome deletion; astrocytoma; dna determination; chromosomes, human, pair 3; hybrid cells; cancer; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; difference analysis |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 92 |
| Issue: | 1 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1995-01-03 |
| Start Page: | 151 |
| End Page: | 155 |
| Language: | English |
| DOI: | 10.1073/pnas.92.1.151 |
| PUBMED: | 7816807 |
| PROVIDER: | scopus |
| PMCID: | PMC42835 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
