DNA and RNA-DNA annealing activity associated with the τ subunit of the Escherichia coil DNA polymerase III holoenzyme Journal Article
| Authors: | Kim, S.; Marians, K. J. |
| Article Title: | DNA and RNA-DNA annealing activity associated with the τ subunit of the Escherichia coil DNA polymerase III holoenzyme |
| Abstract: | The DNA polymerase III (pol III)holoenzyme is the 10 subunit replicase of Escherichia coli. The 71 kDaτ subunit, encoded by dnaX, dimerizes the core polymerase (αεθ) to form pol III' [(αεθ)2τ2]. τ is also a single-stranded DNA-dependent ATPase and can substitute for the γ subunit during initiation complex formation. We show here that τ also possessesa DNA-DNA and RNA-DNA annealing activity that is stimulated by Mg2+ but neither requires ATP nor is inhibited by non-hydrolyzable ATP analogs. This suggests the τ may act to stabilize the primer-template interaction during DNA replication. © 1995 Oxford University Press. |
| Keywords: | controlled study; nonhuman; dna replication; enzyme activity; rna; dna; molecular sequence data; escherichia coli; base sequence; dna sequence; dimerization; primer dna; adenosine triphosphate; enzyme subunit; rna sequence; magnesium ion; nucleic acid renaturation; dna template; polydeoxyribonucleotides; dna directed dna polymerase gamma; adenosine triphosphate derivative; dna polymerase iii; polyribonucleotides; priority journal; article; support, u.s. gov't, p.h.s.; adenosinetriphosphatase; magnesium chloride; adenylyl imidodiphosphate |
| Journal Title: | Nucleic Acids Research |
| Volume: | 23 |
| Issue: | 8 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 1995-04-25 |
| Start Page: | 1374 |
| End Page: | 1379 |
| Language: | English |
| DOI: | 10.1093/nar/23.8.1374 |
| PUBMED: | 7538662 |
| PROVIDER: | scopus |
| PMCID: | PMC306864 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
