Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells Journal Article

   


Authors: Chen, M.; Pan, Z. Q.; Hurwitz, J.
Article Title: Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells
Abstract: Activator 1 (A1; also called replication factor C), in conjunction with proliferating-cell nuclear antigen (PCNA), is essential for the elongation of primed DNA templates by DNA polymerases δ and ε. A1 contains five distinct subunits of 145, 40, 38, 37, and 36.5 kDa. Here we describe the isolation, sequence, and bacterial expression of a cDNA coding for the 40-kDa subunit. In keeping with the presence of an ATP-binding motif, the bacterially expressed 40-kDa subunit binds ATP. The interaction between the 40-kDa subunit and ATP was reduced by the addition of PCNA. In addition, antibodies raised against the 40-kDa subunit abolished the A1-and PCNA-dependent synthesis of DNA catalyzed by polymerase δ. The putative amino acid sequence of the 40-kDa subunit of A1 revealed significant homology with the bacteriophage T4 gene 44 protein and, to a lesser degree, with the τ and γ subunits of Escherichia coli DNA polymerase III holoenzyme.
Keywords: human cell; dna-binding proteins; nonhuman; comparative study; dna replication; dna synthesis; protein analysis; gene expression; hela cell; hela cells; bacteria (microorganisms); dna; amino acid sequence; molecular sequence data; rna, messenger; escherichia coli; recombinant proteins; base sequence; adenosine triphosphate; cycline; protein subunit; sequence homology; dna helicases; dna directed dna polymerase delta; bacteriophage t4; dna polymerase iii; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; proliferating-cell nuclear antigen; elongation factor 1; dna polymerases δ and ε
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 89
Issue: 7
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1992-04-01
Start Page: 2516
End Page: 2520
Language: English
DOI: 10.1073/pnas.89.7.2516
PUBMED: 1313560
PROVIDER: scopus
PMCID: PMC48692
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0026598237&doi=10.1073%2fpnas.89.7.2516&partnerID=40&md5=30923e3e15a2759e1bd20d26b3636b95
Notes: Source: Scopus
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MSK Authors
  1. Jerard Hurwitz
    206 Hurwitz
  2. Zhen-Qiang Pan
    15 Pan
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