Effective targeting of magnetic radioactive (90)Y-microspheres to tumor cells by an externally applied magnetic field. Preliminary in vitro and in vivo results Journal Article
| Authors: | Häfeli, U. O.; Sweeney, S. M.; Beresford, B. A.; Humm, J. L.; Macklis, R. M. |
| Article Title: | Effective targeting of magnetic radioactive (90)Y-microspheres to tumor cells by an externally applied magnetic field. Preliminary in vitro and in vivo results |
| Abstract: | Magnetic biodegradable poly(lactic acid) microspheres that incorporate both magnetite and the β-emitter90Y were prepared. By applying a directional external magnetic field gradient in excess of 0.02 Tesla/cm across a 96-well plate containing neuroblastoma cells incubated with the90Y magnetite loaded microspheres, the radiation dose to the cells could be enhanced or reduced relative to the dose from a uniform loading of the well with90Y-DTPA. Using the MTT assay, cell survival was measured for the magnetic field directed from above (cell sparing) and from below (cell targeting) the well plate, resulting in 65 ± 8% or 18 ± 5% survival respectively. This method was then applied to an in vivo murine tumor model. The biodistribution of intraperitoneally injected magnetic radioactive microspheres, after 24 h in mice, showed that 73 ± 32% of the radioactivity was found on the subcutaneous tumor that had a rare earth magnet fixed above it. In contrast, the tumor radioactivity with no attached magnet was 6 ± 4%. Magnetically targeted radiopolymers such as90Y-microspheres show great promise for regional or intracavitary radiotherapy. © 1995 Elsevier Science Ltd. |
| Keywords: | unclassified drug; human cell; animal cell; mouse; animal; mice; cell survival; skin neoplasms; radiotherapy; cell line; animal experiment; tumor cells, cultured; mice, inbred c57bl; dose-response relationship, radiation; tissue distribution; neuroblastoma; lymphoma; tumor cell; microsphere y 90; yttrium radioisotopes; magnetics; tissue culture; polymers; pentetic acid; microspheres; magnetic field; lactates; human; female; article; support, non-u.s. gov't; pentetate yttrium y 90 |
| Journal Title: | Nuclear Medicine and Biology |
| Volume: | 22 |
| Issue: | 2 |
| ISSN: | 0969-8051 |
| Publisher: | Elsevier Science Inc. |
| Date Published: | 1995-02-01 |
| Start Page: | 147 |
| End Page: | 155 |
| Language: | English |
| DOI: | 10.1016/0969-8051(94)00124-3 |
| PUBMED: | 7767307 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
