In vivo and in vitro studies of cyclophosphamide chemotherapy in a mouse mammary carcinoma by (31)P NMR spectroscopy Journal Article
| Authors: | Street, J. C.; Mahmood, U.; Matei, C.; Koutcher, J. A. |
| Article Title: | In vivo and in vitro studies of cyclophosphamide chemotherapy in a mouse mammary carcinoma by (31)P NMR spectroscopy |
| Abstract: | The effect of cyclophosphamide on the metabolic profile of a mammary carcinoma implanted on the foot of mouse was studied by 31P NMR spectroscopy both in vivo and in perchloric acid extracts. The ratio nucleotide triphosphate: Pi was significantly elevated in cyclophosphamide treated tumours relative to untreated tumours after 96 h in vivo (p<0.05). Phosphocreatine: Pi was similarly elevated from 48 to 168 h (p<0.01). Resolution of the phosphomonoester peak into two distinct resonances allowed us to estimate the ratio of PME′ to phosphocholine (PC), where PME′ is a composite peak consisting, in part, of phosphoethanolamine (PE). PME′:PC was found to be significantly higher in treated animals relative to control animals in vivo (p<0.01 from 48 to 168h). Perchloric acid extract spectra suggest that the increase in PME′:PC was in part due to a decrease in PC concentration and also due to an increase in a previously unidentified resonance which was coresonant with PE. Extract data show that there was a significant increase in the concentration of the phosphodiesters, glycerophosphocholine (p<0.01) and glycerophosphoethanolamine (p<0.05) in treated relative to control tumours. The changes in the phosphomonoester resonances are qualitatively similar to previously described changes following radiation and suggest that they may be a marker of cell kill or lack of cell growth after antineoplastic therapy. Copyright © 1995 John Wiley & Sons, Ltd. |
| Keywords: | dose response; methodology; adenocarcinoma; mouse; animal; metabolism; animals; mice; alkylating agent; cyclophosphamide; drug screening; dose-response relationship, drug; drug screening assays, antitumor; antineoplastic agents, alkylating; magnetic resonance spectroscopy; nuclear magnetic resonance spectroscopy; experimental neoplasm; neoplasm transplantation; phosphate; phosphates; mammary neoplasms, experimental; phospholipids; cancer transplantation; phospholipid; phosphorus; phosphorus isotopes; mice, inbred c3h; c3h mouse; female; article |
| Journal Title: | NMR in Biomedicine |
| Volume: | 8 |
| Issue: | 4 |
| ISSN: | 0952-3480 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1995-06-01 |
| Start Page: | 149 |
| End Page: | 158 |
| Language: | English |
| DOI: | 10.1002/nbm.1940080403 |
| PUBMED: | 8771089 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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