Quantitation of μ-opioid receptor (MOR-1) mRNA in selected regions of the rat CNS Journal Article
| Authors: | Brodsky, M.; Elliott, K.; Hynansky, A.; Jenab, S.; Inturrisi, C. E. |
| Article Title: | Quantitation of μ-opioid receptor (MOR-1) mRNA in selected regions of the rat CNS |
| Abstract: | The μ opioid receptor (MOR-1) mRNA was quantified in rat CNS by a sensitive solution hybridization (SH) technique, employing a 32P-labeled riboprobe derived from the coding region of MOR-1cDNA. In a Northern blot analysis this riboprobe hybridized to a 14 kb form of rat MOR-1 mRNA. The linear range of SH assay extends from 1 to 250 pg of MOR-1 sense transcript (equivalent to 9.3- 2325 pg of MOR-1 mRNA). A microdissection technique for reproducible sampling of selected CNS regions,followed by the SH assay, allowed for a quantitative study of MOR-1 mRNA distribution. The highest levels of MOR-1 mRNA were present in medial thalamus (17.8 ± 0.3 pg/μg RNA), and the lowest in the cerebellum (0.4 ± 0.1 pg/μg RNA). Hypothalamus, dorsal spinal horn, nucleus raphe, periaqueductal gray, and sensorimotor cortex contained intermediate levels. This distribution closely parallels the pattern of μ receptor binding, suggesting that both the mRNA and the receptor protein are colocalized within most of the regions studied. © Rapid Communications of Oxford Ltd. |
| Keywords: | controlled study; nonhuman; sensitivity and specificity; linear models; reproducibility of results; animal; animal tissue; central nervous system; amino acid sequence; molecular sequence data; messenger rna; rna, messenger; spinal cord; rat; reference values; rats; rats, sprague-dawley; mu opiate receptor; brain region; receptors, opioid, mu; northern blotting; rna probes; male; priority journal; article; brain regions; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; adult rat; rna quantitation; solution hybridization; μ opioid receptor mrna |
| Journal Title: | NeuroReport |
| Volume: | 6 |
| Issue: | 5 |
| ISSN: | 0959-4965 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1995-03-27 |
| Start Page: | 725 |
| End Page: | 729 |
| Language: | English |
| DOI: | 10.1097/00001756-199503270-00005 |
| PUBMED: | 7605935 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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