Identification and characterization of three new alternatively spliced μ-opioid receptor isoforms Journal Article

Authors: Pan, Y. X.; Xu, J.; Bolan, E.; Abbadie, C.; Chang, A.; Zuckerman, A.; Rossi, G.; Pasternak, G. W.
Article Title: Identification and characterization of three new alternatively spliced μ-opioid receptor isoforms
Abstract: We have identified four new μ-opioid receptor (MOR)-1 exons, indicating that the gene now contains at least nine exons spanning more than 200 kilobases. Replacement of exon 4 by combinations of the new exons yields three new receptors. When expressed in Chinese hamster ovary cells, all three variants displayed high affinity for μ-opioid ligands, but κ and δ drugs were inactive. However, there were subtle, but significant, differences in the binding profiles of the three variants among themselves and from MOR-1. Immunohistochemically, the major variant, MOR-1C, displayed a regional distribution quite distinct from that of MOR-1. Region-specific processing also was seen at the mRNA level. Antisense mapping revealed that the four new exons were all involved in morphine analgesia. Together with two other variants generated from alternative splicing of exon 4, there are now six distinct MOR-1 receptors.
Keywords: immunohistochemistry; controlled study; exon; exons; nonhuman; mouse; animals; mice; animal tissue; animal experiment; mice, inbred icr; drug receptor binding; cloning, molecular; tissue distribution; brain; rna, messenger; nucleotide sequence; alternative splicing; alternative rna splicing; morphine; analgesia; mu opiate receptor; mu opiate receptor agonist; receptors, opioid, mu; isoprotein; protein isoforms; gene structure; morphine 6 glucuronide; cho cell; cricetinae; brain tissue; enkephalin[2 dextro alanine 4 methylphenylalanine 5 glycine]; intracerebroventricular drug administration; male; priority journal; article
Journal Title: Molecular Pharmacology
Volume: 56
Issue: 2
ISSN: 0026-895X
Publisher: The American Society for Pharmacology and Experimental Therapeutics  
Date Published: 1999-08-01
Start Page: 396
End Page: 403
Language: English
PUBMED: 10419560
PROVIDER: scopus
DOI: 10.1124/mol.56.2.396
Notes: Article -- Export Date: 16 August 2016 -- Source: Scopus
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MSK Authors
  1. Elizabeth A Bolan
    18 Bolan
  2. Catherine Abbadie
    14 Abbadie
  3. Yingxian Pan
    108 Pan
  4. Grace Rossi
    57 Rossi
  5. Gavril W Pasternak
    296 Pasternak
  6. Albert H Chang
    19 Chang
  7. Jin   Xu
    50 Xu