Identification and characterization of three new alternatively spliced μ-opioid receptor isoforms Journal Article
| Authors: | Pan, Y. X.; Xu, J.; Bolan, E.; Abbadie, C.; Chang, A.; Zuckerman, A.; Rossi, G.; Pasternak, G. W. |
| Article Title: | Identification and characterization of three new alternatively spliced μ-opioid receptor isoforms |
| Abstract: | We have identified four new μ-opioid receptor (MOR)-1 exons, indicating that the gene now contains at least nine exons spanning more than 200 kilobases. Replacement of exon 4 by combinations of the new exons yields three new receptors. When expressed in Chinese hamster ovary cells, all three variants displayed high affinity for μ-opioid ligands, but κ and δ drugs were inactive. However, there were subtle, but significant, differences in the binding profiles of the three variants among themselves and from MOR-1. Immunohistochemically, the major variant, MOR-1C, displayed a regional distribution quite distinct from that of MOR-1. Region-specific processing also was seen at the mRNA level. Antisense mapping revealed that the four new exons were all involved in morphine analgesia. Together with two other variants generated from alternative splicing of exon 4, there are now six distinct MOR-1 receptors. |
| Keywords: | immunohistochemistry; controlled study; exon; exons; nonhuman; mouse; animals; mice; animal tissue; animal experiment; mice, inbred icr; drug receptor binding; cloning, molecular; tissue distribution; brain; rna, messenger; nucleotide sequence; alternative splicing; alternative rna splicing; morphine; analgesia; mu opiate receptor; mu opiate receptor agonist; receptors, opioid, mu; isoprotein; protein isoforms; gene structure; morphine 6 glucuronide; cho cell; cricetinae; brain tissue; enkephalin[2 dextro alanine 4 methylphenylalanine 5 glycine]; intracerebroventricular drug administration; male; priority journal; article |
| Journal Title: | Molecular Pharmacology |
| Volume: | 56 |
| Issue: | 2 |
| ISSN: | 0026-895X |
| Publisher: | The American Society for Pharmacology and Experimental Therapeutics |
| Date Published: | 1999-08-01 |
| Start Page: | 396 |
| End Page: | 403 |
| Language: | English |
| PUBMED: | 10419560 |
| PROVIDER: | scopus |
| DOI: | 10.1124/mol.56.2.396 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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