Sensitive detection of prostatic hematogenous tumor cell dissemination using prostate specific antigen and prostate specific membrane-derived primers in the polymerase chain reaction Journal Article

  

Authors:	Israeli, R. S.; Miller, W. H. Jr; Su, S. L.; Samadi, D. S.; Powell, C. T.; Heston, W. D. W.; Wise, G. J.; Fair, W. R.
Article Title:	Sensitive detection of prostatic hematogenous tumor cell dissemination using prostate specific antigen and prostate specific membrane-derived primers in the polymerase chain reaction
Abstract:	We developed a polymerase chain reaction based assay enabling sensitive detection of hematogenous tumor cell dissemination in patients with prostate cancer. We performed 'nested polymerase chain reaction,' amplifying messenger ribonucleic acid sequences unique to prostate specific antigen (PSA) and to the prostate specific membrane antigen, and compared the respective results. Prostatic tumor cells were detected in 2 of 30 patients (6.7%) by polymerase chain reaction with PSA derived primers, while prostate specific membrane primers detected tumor cells in 19 (63.3%). All 16 negative controls had negative PSA and prostate specific membrane polymerase chain reaction. Assays were repeated to confirm results, and polymerase chain reaction products were verified by deoxyribonucleic acid sequencing and Southern analysis. Patients harboring circulating prostatic tumor cells as detected by prostate specific membrane and not by PSA polymerase chain reaction included 7 of 13 previously treated by radical prostatectomy who had nonmeasurable serum PSA levels at the time of this assay. The significance of these findings with respect to future disease recurrence and progression will be investigated.
Keywords:	human tissue; cancer recurrence; polymerase chain reaction; prostate specific antigen; metastasis; prostate cancer; membrane antigen; prostatectomy; prostate tumor; diagnosis; dna sequence; rna sequence; southern blotting; rna directed dna polymerase; human; male; priority journal; article
Journal Title:	Journal of Urology
Volume:	153
Issue:	3
ISSN:	0022-5347
Publisher:	Elsevier Science, Inc.
Date Published:	1995-03-01
Start Page:	573
End Page:	577
Language:	English
DOI:	10.1016/s0022-5347(01)67651-1
PUBMED:	7532229
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028854977&doi=10.1016%2fS0022-5347%2801%2967651-1&partnerID=40&md5=3f60a29b4418275aa469526cdcc169f1
Notes:	Article -- Export Date: 28 August 2018 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/131432