Establishment and characterization of a novel hepatoblastoma-derived cell line Journal Article
| Authors: | Manchester, K. M.; Warren, D. J.; Erlandson, R. A.; Wheatley, J. M.; La Quaglia, M. P. |
| Article Title: | Establishment and characterization of a novel hepatoblastoma-derived cell line |
| Abstract: | Hepatoblastoma is the most common pediatric liver cancer, and complete resection is required for long-term survival. There are few reported hepatoblastoma cell lines available for study. In order to develop in vitro and animal models, the authors isolated an additional cell line (HB1) from a human hepatoblastoma and here report its characteristics in culture. Cells were analyzed for growth rate, clonigenicity, and tumorigenicity, using electron microscopy. Immunocytochemistry and Northern analysis for the expression of specific surface markers and genes of interest were also performed. HB1 cells required fetal calf serum but grew well in culture (population doubling time of 2 days) and had an undifferentiated appearance under electron microscopy. Epidermal growth factor (EGF) and hydrocortisone stimulated growth with or without serum, but no autocrine growth stimulation via the epidermal growth factor receptor was detected. Proteins produced by HB1 cells under normal culture conditions included α-fetoprotein, cytokeratins 8 and 18, and lactate dehydrogenase (with an isozyme subunit composition similar to that of liver). HB1 cells showed chronic, high expression of c-myc and Ha-ras oncogenes but no N-myc and apparently normal RB gene expression. This cell line shows characteristics in culture consistent with malignant, hepatic epithelial cells and is apparently EGF dependent. It may provide a model system for the future development of alternative therapies. © 1995. |
| Keywords: | epidermal growth factor; controlled study; human tissue; human cell; cisplatin; doxorubicin; nonhuman; liver neoplasms; mouse; electron microscopy; microscopy, electron; animal tissue; gene expression; cell growth; culture medium; cell line; animal experiment; cyclophosphamide; cancer cell culture; tumor cells, cultured; oncogene h ras; monoclonal antibody; gene expression regulation, neoplastic; infant; immunocytochemistry; carcinogenicity; cell isolation; lactate dehydrogenase; cytokeratin; oncogene c myc; blotting, northern; model; alpha fetoprotein; growth rate; northern blotting; fetal calf serum; tumor stem cell assay; polyclonal antibody; hepatoblastoma; human; male; priority journal; article; hydrocortisone sodium succinate |
| Journal Title: | Journal of Pediatric Surgery |
| Volume: | 30 |
| Issue: | 4 |
| ISSN: | 0022-3468 |
| Publisher: | W.B. Saunders Co-Elsevier Inc. |
| Date Published: | 1995-04-01 |
| Start Page: | 553 |
| End Page: | 558 |
| Language: | English |
| DOI: | 10.1016/0022-3468(95)90129-9 |
| PUBMED: | 7595832 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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19Warren
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