Neoplastic transformation of mouse mammary epithelial cells by deregulated myc expression Journal Article

   


Authors: Telang, N. T.; Osborne, M. P.; Sweterlitsch, L. A.; Narayanan, R.
Article Title: Neoplastic transformation of mouse mammary epithelial cells by deregulated myc expression
Abstract: A spontaneously immortalized, nontumorigenic mouse mammary epithelial cell line (MMEC) was transfected with an activated myc construct by electroporation. Constitutive expression of myc in MMEC resulted in anchorage independence in soft agar and tumorigenicity in nude mice. The myc-expressing MMEC showed higher saturation density, faster growth rate, and partial abrogation of serum-derived growth factor(s) requirement compared with parent MMEC. Epidermal growth factor or transforming growth factor alpha stimulated the anchorage-independent growth, but not the anchorage-dependent growth, of MMEC-myc cells. Type 1 transforming growth factor beta, on the other hand, inhibited both the anchorage-independent and anchorage-dependent growth of MMEC-myc cells. These results demonstrate that deregulated expression of myc results in neoplastic transformation in mammary epithelial cells. Accompanying the transformation is altered sensitivity to polypeptide growth factors. © 1990 by The American Society for Cell Biology.
Keywords: epidermal growth factor; genetics; mouse; animal; mice; transforming growth factor beta; cell line; transfection; cell transformation, neoplastic; gene expression regulation; gene expression regulation, neoplastic; genetic transfection; nude mouse; mice, nude; cell transformation; genes, myc; neoplasm transplantation; epithelium; proto-oncogene proteins c-myc; isolation and purification; udder; oncogene myc; transforming growth factor alpha; cancer transplantation; female; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; mammae; protein c myc; proto oncogene proteins c myc
Journal Title: Cell Regulation
Volume: 1
Issue: 11
ISSN: 1044-2030
Publisher: The American Society for Cell Biology  
Date Published: 1990-10-01
Start Page: 863
End Page: 872
Language: English
DOI: 10.1091/mbc.1.11.863
PUBMED: 2088530
PROVIDER: scopus
PMCID: PMC362853
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025504571&doi=10.1091%2fmbc.1.11.863&partnerID=40&md5=a681269bed4acd7a25ba972ce4ba89c2
Notes: Article -- Export Date: 27 January 2020 -- Source: Scopus
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MSK Authors
  1. Michael P. Osborne
    61 Osborne
  2. Nitin Telang
    29 Telang
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