Detection of circulating tumor cells in patients with localized and metastatic prostatic carcinoma: Clinical implications Journal Article
| Authors: | Ghossein, R. A.; Scher, H. I.; Gerald, W. L.; Kelly, W. K.; Curley, T.; Amsterdam, A.; Zhang, Z. F.; Rosai, J. |
| Article Title: | Detection of circulating tumor cells in patients with localized and metastatic prostatic carcinoma: Clinical implications |
| Abstract: | Purpose: To determine the frequency with which prostate-specific antigen (PSA)-positive cells can be detected in the peripheral blood of patients with prostatic cancer in different stages and with different sensitivities to hormonal therapy. Patients and Methods: Peripheral blood from 107 men with prostatic cancer and 27 non-prostate cancer controls was analyzed for PSA mRNA using reverse-transcriptase polymerase chain reaction (RT-PCR) and Southern blotting. Results: The lower limit of detection was one PSA- producing cell diluted into 1 x 106 blood mononuclear cells. The test detected PSA mRNA in four of 25 patients (16%) with clinically organ-confined (T1-2) disease, three of 10 (30%) with T3-4 or N+ tumors, and 25 of 72 (35%) with distant metastases. None of the control samples were positive. An increase in positivity was observed with increasing PSA levels. Within the subgroup of patients with distant metastases, positivity was observed in six of 16 patients (38%) with normal or undetectable PSA levels after hormonal therapy and, overall, in 37% of patients (21 of 57) with androgen-independent disease. Conclusion: An RT-PCR-based assay for PSA mRNA can detect circulating cells in the peripheral blood of patients with prostatic cancer. The frequency of positivity increases with tumor stage. A unique observation was the detection of cells in patients with no measurable PSA on hormonal therapy. This suggests that continued seeding of distant sites may still be occurring in these patients, despite seemingly successful therapy. The relationship between continued seeding, disease progression, and survival will require further study. |
| Keywords: | cancer survival; major clinical study; cancer localization; cancer staging; nuclear magnetic resonance imaging; cancer diagnosis; prostate specific antigen; metastasis; reverse transcription polymerase chain reaction; hormonal therapy; prostate carcinoma; human; male; priority journal; article |
| Journal Title: | Journal of Clinical Oncology |
| Volume: | 13 |
| Issue: | 5 |
| ISSN: | 0732-183X |
| Publisher: | American Society of Clinical Oncology |
| Date Published: | 1995-05-01 |
| Start Page: | 1195 |
| End Page: | 1200 |
| Language: | English |
| DOI: | 10.1200/jco.1995.13.5.1195 |
| PUBMED: | 7537803 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work