NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center Journal Article
| Authors: | Wang, S.; Sakai, H.; Wiedmann, M. |
| Article Title: | NAC covers ribosome-associated nascent chains thereby forming a protective environment for regions of nascent chains just emerging from the peptidyl transferase center |
| Abstract: | We demonstrate that nascent polypeptide-associated complex (NAC) is one of the first cytosolic factors that newly synthesized nascent chains encounter. When NAC is present, nascent chains are segregated from the cytosol until ~30 amino acids in length, a finding consistent with the well-documented protease resistance of short ribosome-associated nascent chains. When NAC is removed, the normally protected nascent chains are susceptible to proteolysis. Therefore NAC, by covering COOH-terminal segments of nascent chains on the ribosome, perhaps together with ribosomal proteins, forms a protective environment for regions of nascent chains just emerging from the peptidyl transferase center. Since NAG is not a core ribosomal protein, the emergence of nascent chains from the ribosome may be more dynamic than previously thought. |
| Keywords: | controlled study; nonhuman; proteins; animal; protein degradation; molecular dynamics; protein binding; amino acid sequence; molecular sequence data; peptide fragments; base sequence; cytosol; peptide synthesis; ribosomal proteins; ribosomes; peptidyltransferase; rabbits; ribosome; cross-linking reagents; translation regulation; cell-free system; seeds; factor xa; reticulocytes; priority journal; article; peptide chain elongation; peptide chain initiation |
| Journal Title: | Journal of Cell Biology |
| Volume: | 130 |
| Issue: | 3 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1995-08-01 |
| Start Page: | 519 |
| End Page: | 528 |
| Language: | English |
| DOI: | 10.1083/jcb.130.3.519 |
| PUBMED: | 7622554 |
| PROVIDER: | scopus |
| PMCID: | PMC2120527 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
