13,14-dihydroxy-retinol, a new bioactive retinol metabolite Journal Article

  


Authors: Derguini, F.; Nakanishi, K.; Hämmerling, U.; Chua, R.; Eppinger, T.; Levi, E.; Buck, J.
Article Title: 13,14-dihydroxy-retinol, a new bioactive retinol metabolite
Abstract: Deprivation of vitamin A (retinol) leads to reduced potential of B cell proliferation and nearly complete block of T cell activation in vitro. Retinol, which is thought to function as a pro-hormone, is enzymatically converted into intracellular messenger molecules. Thus, 14-hydroxy-retro- retinol (14-HRR) is an intracellular messenger molecule linked to activation and growth regulation of lymphocytes; whereas, anhydroretinol, another natural retroretinoid, is an antagonist of 14-HRR effects. In this article, we describe the isolation, structure determination, synthesis, and biological properties of a new intracellular retinol derivative, 13,14-dihydroxy- retinol (DHR), which also supports the viability of retinol-deprived lymphocytes. DHR is found in numerous cell lines representing a large cross- section of tissues and animals from insects to mammals. In T lymphocytes the production of DHR and 14-HRR is up-regulated by phorbol ester. DHR is converted to 14-HRR by mild acid treatment, but not by cells; therefore DHR is not a biosynthetic intermediate in the conversion of retinol to 14-HRR. DHR is a distinct end point of retinol metabolism. Although it is linked to cell proliferation, its biological role remains to be determined.
Keywords: human cell; cell proliferation; protein analysis; cell line; b lymphocyte; protein synthesis; growth regulation; magnetic resonance spectroscopy; protein structure; retinol; retinol deficiency; t lymphocyte activation; protein isolation; vitamin a; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Journal of Biological Chemistry
Volume: 270
Issue: 32
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 1995-08-11
Start Page: 18875
End Page: 18880
Language: English
DOI: 10.1074/jbc.270.32.18875
PUBMED: 7642543
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028978697&doi=10.1074%2fjbc.270.32.18875&partnerID=40&md5=6aa88fbb0698e0553ac1f62c95f9657e
Notes: Article -- Export Date: 28 August 2018 -- Source: Scopus
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MSK Authors
  1. Ramon Chua
    21 Chua
  2. Ulrich Hammerling
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  3. Ester Levi
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