Characterization of a 1.8-kb DNA segment located upstream from the human Ha-ras protooncogene and possibly regulating its function Journal Article
| Authors: | Chen, W.; Ruml, T.; Steinberg, M. L. |
| Article Title: | Characterization of a 1.8-kb DNA segment located upstream from the human Ha-ras protooncogene and possibly regulating its function |
| Abstract: | We have cloned a 1.8-kb segment of DNA just adjacent to the 5′ end of the 6.4-kb BamHI fragment which contains the Ha-ras oncogene (rasl; GenBank notation HUMRASH). Electrophoretic mobility shift assays (EMSA) indicate the presence of multiple nuclear protein-binding sites within the 1.8-kb segment. At least three putative regulatory protein-binding sites have been identified in a 206-bp subfragment by DNase I footprint analysis. Within the subfragment containing the DNase-protected regions, there is also a segment containing a number of consensus sequences for DNA-binding proteins and two sub-sequences that exhibit strong sequence homology to at least two previously characterized enhancers. These data suggest that a novel set of regulatory elements may lie as far as 2 kb upstream from the normal Ha-ras transcription start points. © 1995. |
| Keywords: | sequence analysis; proto oncogene; transcription, genetic; hela cells; oncogene h ras; cloning, molecular; amino acid sequence; molecular sequence data; cell line, transformed; base sequence; gene control; genes, ras; keratinocytes; gene location; dna fragment; deoxyribonuclease; enhancer region; enhancer; genetic regulation; dna footprinting; genomic library; human; priority journal; article; support, u.s. gov't, p.h.s.; nuclear factors; dnase i footprint; gel-shift |
| Journal Title: | Gene |
| Volume: | 155 |
| Issue: | 2 |
| ISSN: | 0378-1119 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1995-04-03 |
| Start Page: | 253 |
| End Page: | 256 |
| Language: | English |
| DOI: | 10.1016/0378-1119(94)00767-m |
| PUBMED: | 7721100 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
