Cloning and characterization of a mouse σ1 receptor Journal Article
| Authors: | Pan, Y. X.; Mei, J.; Xu, J.; Wan, B. L.; Zuckerman, A.; Pasternak, G. W. |
| Article Title: | Cloning and characterization of a mouse σ1 receptor |
| Abstract: | A cDNA clone (S2-1a) isolated from a mouse brain cDNA library, using a guinea pig σ1 cDNA as probe, has high homology to the predicted protein sequence of the guinea pig (88%) and human (90%) σ1 receptors. Northern analysis revealed a major mRNA of ~1.8 kb in a wide range of mouse tissues, with highest levels in brain, liver, kidney, and thymus. Southern analysis and chromosomal mapping in the mouse suggested a single-copy gene in region A5-B2 of chromosome 4. Expression of the clone in MCF-7 and CHO cells led to a pronounced increase in (+)-[3H]pentazocine binding with a selectivity profile consistent with σ1 receptors. In vitro translation yielded a protein of ~28 kDa, as did transfection of a probe containing the hemagglutinin (HA) epitope (S2-1a.HA) into CHO cells, as determined by western analysis using an antibody directed against HA. (+)-[3H]-Pentazocine binding to immunopurified HA-tagged receptor demonstrated conclusively that S2-1a.HA encodes a high-affinity (+)-[3H]pentazocine binding site with characteristics of a murine σ1 receptor. An antisense oligodeoxynucleotide designed from S2-1a potentiated opioid analgesia in vivo. |
| Keywords: | nonhuman; polymerase chain reaction; animal cell; mouse; animals; mice; animal tissue; pain; gene expression; tumor cells, cultured; gene library; animalia; drug receptor binding; molecular cloning; cloning, molecular; amino acid sequence; molecular sequence data; brain; recombinant proteins; organ specificity; murinae; rodentia; analgesics, opioid; pain measurement; opioid; sequence homology; analgesia; chromosome map; chromosome mapping; oligonucleotides, antisense; cho cells; cricetinae; cloning; pentazocine; antisense; gene isolation; guinea pig; narcotic antagonists; guinea pigs; humans; human; male; priority journal; article; sus scrofa; cavia porcellus; mink cell focus-forming virus; sigma opiate receptor; σ receptor; antiopioid |
| Journal Title: | Journal of Neurochemistry |
| Volume: | 70 |
| Issue: | 6 |
| ISSN: | 0022-3042 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1998-06-01 |
| Start Page: | 2279 |
| End Page: | 2285 |
| Language: | English |
| PUBMED: | 9603192 |
| PROVIDER: | scopus |
| DOI: | 10.1046/j.1471-4159.1998.70062279.x |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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