Ganglioside mediate the interaction between Nogo receptor 1 and LINGO-1 Journal Article

Authors: Saha, N.; Kolev, M. V.; Semavina, M.; Himanen, J.; Nikolov, D. B.
Article Title: Ganglioside mediate the interaction between Nogo receptor 1 and LINGO-1
Abstract: Upon spinal cord injury, the myelin inhibitors, including the myelin-associated glycoprotein (MAG), Nogo-A and the oligodendrocyte myelin glycoprotein (OMgp), bind to and signal via a single neuronal receptor/co-receptor complex comprising of Nogo receptor 1(NgR1)/LINGO-1 and p75 or TROY, impeding regeneration of injured axons. We employed a cell-free system to study the binding of NgR1 to its co-receptors and the myelin inhibitor Nogo-A, and show that gangliosides mediate the interaction of NgR1 with LINGO-1. Solid phase binding assays demonstrate that the sialic acid moieties of gangliosides and the stalk of NgR1 are the principal determinants of these molecular interactions. Moreover, the tripartite complex comprising of NgR1, LINGO-1 and ganglioside exhibits stronger binding to Nogo-A (Nogo-54) in the presence of p75, suggesting the gangliosides modulate the myelin inhibitor-receptor signaling. © 2011 Elsevier Inc.
Keywords: ganglioside; nogo receptor; nogo-a; lingo-1; p75
Journal Title: Biochemical and Biophysical Research Communications
Volume: 413
Issue: 1
ISSN: 0006-291X
Publisher: Elsevier Science, Inc.  
Date Published: 2011-09-16
Start Page: 92
End Page: 97
Language: English
DOI: 10.1016/j.bbrc.2011.08.060
PROVIDER: scopus
PUBMED: 21872576
Notes: --- - "Export Date: 3 October 2011" - "CODEN: BBRCA" - "Source: Scopus"
Altmetric Score
MSK Authors
  1. Dimitar B Nikolov
    70 Nikolov
  2. Juha P Himanen
    42 Himanen
  3. Nayanendu Saha
    14 Saha
  4. Momchil V Kolev
    7 Kolev