Inhibition of ligand-induced activation of epidermal growth factor receptor tyrosine phosphorylation by curcumin Journal Article
| Authors: | Korutla, L.; Cheung, J. Y.; Mendelsohn, J.; Kumar, R. |
| Article Title: | Inhibition of ligand-induced activation of epidermal growth factor receptor tyrosine phosphorylation by curcumin |
| Abstract: | We explored the regulation of epidermal growth factor (EGF)-mediated activation of EGF receptor (EGF-R) phosphorylation by curcumin (diferuloyl-methane), a recently identified kinase inhibitor, in cultured NIH 3T3 cells expressing human EGF-R. Treatment of cells with a saturating concentration of EGF for 5-15 min induced increased EGF-R tyrosine phosphorylation by 4- to 11-fold and this was inhibited in a dose- and time-dependent manner by up to 90% by curcumin, which also inhibited the growth of EGF-stimulated cells. There was no effect of curcumin treatment on the amount of surface expression of labeled EGF-R and inhibition of EGF-mediated tyrosine phosphorylation of EGF-R by curcumin was mediated by a reversible mechanism. In addition, curcumin also inhibited EGF-induced, but not bradykinin-induced, calcium release. These findings demonstrate that curcumin is a potent inhibitor of a growth stimulatory pathway, the ligand-induced activation of EGF-R, and may potentially be useful in developing anti-proliferative strategies to control tumor cell growth. © 1995 Oxford University Press. |
| Keywords: | epidermal growth factor; nonhuman; animal cell; mouse; animal; mice; cell division; epidermal growth factor receptor; culture medium; calcium; receptor, epidermal growth factor; enzyme activation; phosphorylation; cancer inhibition; enzyme phosphorylation; cell culture; ligand; immunoprecipitation; immunoblotting; cell strain 3t3; epidermal growth factor receptor kinase; curcumin; 3t3 cells; polyacrylamide gel electrophoresis; calcium transport; growth stimulation; priority journal; article; support, non-u.s. gov't |
| Journal Title: | Carcinogenesis |
| Volume: | 16 |
| Issue: | 8 |
| ISSN: | 0143-3334 |
| Publisher: | Oxford University Press |
| Date Published: | 1995-08-01 |
| Start Page: | 1741 |
| End Page: | 1745 |
| Language: | English |
| DOI: | 10.1093/carcin/16.8.1741 |
| PUBMED: | 7634398 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
