Transforming growth factor ß modulates phosphorylation of the epidermal growth factor receptor and proliferation of A431 cells Journal Article


Authors: Goldkorn, T.; Mendelsohn, J.
Article Title: Transforming growth factor ß modulates phosphorylation of the epidermal growth factor receptor and proliferation of A431 cells
Abstract: Transforming growth factor beta (TGF-beta) increased the phosphorylation of the epidermal growth factor (EGF) receptor and inhibited the growth of A431 cells. Incubation with TGF-beta induced maximal EGF receptor phosphorylation to levels 1.5-fold higher than controls. Phosphorylation increased more prominently (4-5-fold) on tyrosine residues as determined by phosphoamino acid analysis and antiphosphotyrosine antibody immunoblotting. The kinase activity of EGF receptor was also elevated 2.5-fold when cells were cultured in the presence of TGF-beta. The antiproliferative effect of TGF-beta on A431 cells was accompanied by prolongation of G0-G1 phase and by morphological changes. TGF-beta augmented the growth inhibition of A431 cells which could be induced by EGF. In parallel, the specific EGF-induced increase in total phosphorylation of the EGF receptor was also augmented in the presence of TGF-beta. In cells cultured with TGF-beta, the phosphorylation of EGF receptor tyrosines induced by 20-min exposure to EGF was further increased 2-3-fold, suggesting additive effects upon receptor phosphorylation. EGF receptor activation by TGF-beta is characterized by kinetics quite distinct from that induced by EGF and therefore appears to take place through an independent mechanism. The TGF-beta-induced elevation in the phosphorylation of the EGF receptor may have a role in the augmented growth inhibition of A431 cells observed in the presence of EGF and TGF-beta.
Keywords: signal transduction; epidermal growth factor; comparative study; metabolism; cell division; transforming growth factor beta; epidermal growth factor receptor; receptor, epidermal growth factor; drug effect; tumor cells, cultured; tyrosine; phosphorylation; cell culture; half life time; cell cycle g1 phase; g1 phase; cell cycle g0 phase; half-life; g0 phase; humans; human; article
Journal Title: Cell Growth & Differentiation
Volume: 3
Issue: 2
ISSN: 1044-9523
Publisher: American Association for Cancer Research  
Date Published: 1992-02-01
Start Page: 101
End Page: 109
Language: English
PUBMED: 1504015
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 30 July 2019 -- Source: Scopus
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