Altered patterns of retinoblastoma gene product expression in adult soft-tissue sarcomas Journal Article
| Authors: | Karpeh, M. S.; Brennan, M. F.; Cance, W. G.; Woodruff, J. M.; Pollack, D.; Casper, E. S.; Dudas, M. E.; Latres, E.; Drobnjak, M.; Cordon-Cardo, C. |
| Article Title: | Altered patterns of retinoblastoma gene product expression in adult soft-tissue sarcomas |
| Abstract: | Altered expression of the retinoblastoma (RB) tumour-suppressor gene product (pRB) has been detected in sporadic bone and soft-tissue sarcomas. Earlier studies, analysing small cohorts of sarcoma patients, have suggested that these alterations are more commonly associated with high-grade tumours, metastatic lesions and poorer survival. This study was designed to re-examine the prevalence and clinical significance of altered pRB expression in a large and selected group of soft-tissue sarcomas from 174 adult patients. Representative tissue sections from these sarcomas were analysed by immunohistochemistry using a well-characterised anti-pRB monoclonal antibody. Tumours were considered to have a positive pRB phenotype only when pure nuclear staining was demonstrated, and cases were segregated into one of three groups. Group 1 (n = 36) were patients whose tumours have minimal or undetectable pRB nuclear staining (≪ 20% of tumour cells) and were considered pRB negative. Patients with tumours staining in a heterogeneous pattern (20-79% of tumour cells) were classified as group 2 (n = 99). The staining of group 3 (n = 39) was strongly positive with a homogeneous pRB nuclear immunoreactivity (80-100% of tumour cells). pRB alterations were frequently observed in both low- and high-grade lesions. Altered pRB expression did not correlate with known predictors of survival and was not itself an independent predictor of outcome in the long-term follow-up. These findings support earlier observations that alterations of pRB expression are common events in soft-tissue sarcomas; nevertheless, long-term follow-up results indicate that altered patterns of pRB expression do not influence clinical outcome of patients affected with soft-tissue sarcomas. It is postulated that RB alterations are primary events in human sarcomas and may be involved in tumorigenesis or early phases of tumour progression in these neoplasias. © 1995 Stockton Press. All rights reserved. |
| Keywords: | immunohistochemistry; osteosarcoma; adult; cancer survival; controlled study; human tissue; survival rate; major clinical study; follow up; cancer grading; phenotype; metastasis; gene expression; prevalence; retinoblastoma; immunoreactivity; carcinogenesis; monoclonal antibody; sarcoma; tumor suppressor gene; soft tissue sarcoma; tumor growth; retinoblastoma protein; soft tissue neoplasms; middle age; genes, retinoblastoma; human; male; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | British Journal of Cancer |
| Volume: | 72 |
| Issue: | 4 |
| ISSN: | 0007-0920 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1995-10-01 |
| Start Page: | 986 |
| End Page: | 991 |
| Language: | English |
| DOI: | 10.1038/bjc.1995.447 |
| PUBMED: | 7547254 |
| PROVIDER: | scopus |
| PMCID: | PMC2034041 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work