Identification of FAM46D as a novel cancer/testis antigen using EST data and serological analysis Journal Article
| Authors: | Bettoni, F.; Filho, F. C.; Grosso, D. M.; Galante, P. A. F.; Parmigiani, R. B.; Geraldo, M. V.; Henrique-Silva, F.; Oba-Shinjo, S. M.; Marie, S. K. N.; Soares, F. A.; Brentani, H. P.; Simpson, A. J. G.; de Souza, S. J.; Camargo, A. A. |
| Article Title: | Identification of FAM46D as a novel cancer/testis antigen using EST data and serological analysis |
| Abstract: | Cancer/testis Antigens (CTAs) are immunogenic proteins with a restricted expression pattern in normal tissues and aberrant expression in different types of tumors being considered promising candidates for immunotherapy. We used the alignment between EST sequences and the human genome sequence to identify novel CT genes. By examining the EST tissue composition of known CT clusters we defined parameters for the selection of 1184 EST clusters corresponding to putative CT genes. The expression pattern of 70 CT gene candidates was evaluated by RT-PCR in 21 normal tissues, 17 tumor cell lines and 160 primary tumors. We were able to identify 4 CT genes expressed in different types of tumors. The presence of antibodies against the protein encoded by 1 of these 4 CT genes (FAM46D) was exclusively detected in plasma samples from cancer patients. Due to its restricted expression pattern and immunogenicity FAM46D represents a novel target for cancer immunotherapy. © 2009 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; unclassified drug; gene cluster; human cell; drug targeting; cancer patient; colorectal cancer; cancer immunotherapy; reverse transcription polymerase chain reaction; gene expression profiling; lung cancer; cancer cell culture; immunotherapy; blood sampling; sequence alignment; ests; fam46d; tumor antigens; cancer testis antigen; family with sequence similarity 46 member d; melanoma antigen 1; melanoma antigen 10; melanoma antigen 3; ny eso 1 antigen; transcription factor e2f; antigen detection; blood analysis; expressed sequence tag; gene identification; glioblastoma; human genome; immunogenicity; nucleotide sequence; uterine cervix cancer |
| Journal Title: | Genomics |
| Volume: | 94 |
| Issue: | 3 |
| ISSN: | 0888-7543 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2009-09-01 |
| Start Page: | 153 |
| End Page: | 160 |
| Language: | English |
| DOI: | 10.1016/j.ygeno.2009.06.001 |
| PROVIDER: | scopus |
| PUBMED: | 19540335 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 3" - "Export Date: 30 November 2010" - "CODEN: GNMCE" - "Source: Scopus" |
