Reprogramming to a muscle fate by fusion recapitulates differentiation Journal Article
| Authors: | Pomerantz, J. H.; Mukherjee, S.; Palermo, A. T.; Blau, H. M. |
| Article Title: | Reprogramming to a muscle fate by fusion recapitulates differentiation |
| Abstract: | Fusion of mammalian cells to form stable, non-dividing heterokaryons results in nuclear reprogramming without the exchange of genetic material. In this report, we show that reprogramming in somatic cell heterokaryons involves activation of the canonical skeletal muscle transcription factors as well as contraction-excitation genes. Thus, the effect of heterokaryon formation on gene expression is to induce a recapitulation of differentiation. Heterokaryons formed with a relatively refractory cell type, the hepatocyte cell line HepG2, revealed the importance of both MyoD expression and other unidentified cytoplasmic components, neither of which are sufficient for efficient muscle gene activation, but are synergistic. We provide evidence that de-repression by transient histone deacetylase inhibition can induce MyoD expression and increase the extent and efficiency of muscle gene transcription. Taken together, the results suggest that understanding the mechanistic basis, using a combination of approaches, and taking into account cell history, will facilitate an increase in the efficiency and fidelity of conversion from one differentiated phenotype to another desired cell type. Inherent advantages of the heterokaryon system merit further investigation in the pursuit of directed cloning. |
| Keywords: | controlled study; protein expression; genetics; nonhuman; animal cell; mouse; animal; cytology; metabolism; mammalia; animals; mice; enzyme inhibition; gene expression; genetic transcription; cell differentiation; drug effect; enzymology; enzyme inhibitor; cell line, tumor; keratinocyte; cell lineage; cell type; gene expression regulation; gene activation; drug antagonism; species specificity; enzyme inhibitors; oligonucleotide array sequence analysis; gene repression; tumor cell line; cytoplasm; species difference; dna microarray; keratinocytes; histone deacetylases; histone deacetylase; nuclear reprogramming; excitation contraction coupling; cell fusion; myoblast; fusion; heterokaryon; histone deacetylation; myod; reprogramming; myod protein; cell strain hepg2; muscle; muscles; myoblasts |
| Journal Title: | Journal of Cell Science |
| Volume: | 122 |
| Issue: | 7 |
| ISSN: | 0021-9533 |
| Publisher: | Company of Biologists |
| Date Published: | 2009-01-01 |
| Start Page: | 1045 |
| End Page: | 1053 |
| Language: | English |
| DOI: | 10.1242/jcs.041376 |
| PUBMED: | 19295131 |
| PROVIDER: | scopus |
| PMCID: | PMC2720934 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 30 November 2010" - "CODEN: JNCSA" - "Source: Scopus" |
