A novel host factor for integration of mycobacteriophage L5 Journal Article
| Authors: | Pedulla, M. L.; Lee, M. H.; Lever, D. C.; Hatfull, G. F. |
| Article Title: | A novel host factor for integration of mycobacteriophage L5 |
| Abstract: | Bacterial integration host factors (IHFs) play central roles in the cellular processes of recombination, DNA replication, transcription, and bacterial pathogenesis. We describe here a novel mycobacterial IHF (mIHF) of Mycobacterium smegmatis and Mycobacterium tuberculosis that stimulates integration of mycobacteriophage L5. mIHF is the product of a single gene and is unrelated at the sequence level to other integration host factors. By itself, mIHF does not bind preferentially to attP DNA, although it significantly alters the pattern of integrase (Int) binding, promoting the formation of specific integrase-mIHF-attP intasome complexes. |
| Keywords: | gene sequence; dna-binding proteins; nonhuman; mycobacterium tuberculosis; bacterial proteins; amino acid sequence; molecular sequence data; sequence homology, amino acid; recombination, genetic; base sequence; mycobacterium; mycobacterium smegmatis; virus gene; dna footprinting; bacteriophage; virus dna cell dna interaction; genes, viral; deoxyribonuclease i; priority journal; article; integration host factors; mycobacteriophages |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 93 |
| Issue: | 26 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1996-12-24 |
| Start Page: | 15411 |
| End Page: | 15416 |
| Language: | English |
| PUBMED: | 8986825 |
| PROVIDER: | scopus |
| PMCID: | PMC26418 |
| DOI: | 10.1073/pnas.93.26.15411 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
