Characterization of the mycobacteriophage L5 attachment site, attP Journal Article
| Authors: | Peña, C. E. A.; Lee, M. H.; Pedulla, M. L.; Hatfull, G. F. |
| Article Title: | Characterization of the mycobacteriophage L5 attachment site, attP |
| Abstract: | Lysogenization of mycobacteriophage L5 involves integration of the phage genome into the Mycobacterium smegmatis chromosome. Integration occurs by a site-specific recombination event between a phage attachment site, attP, and a bacterial attachment site, attB, which is catalyzed by the phage-encoded integrase protein. DNase I footprinting reveals that L5 integrase binds to two types of sites within attP which span an unexpectedly large region of 413 bp: seven arm-type sites (P1 to P7) each of which correspond to a consensus sequence 5'-TGCaaCtcYy, and core-type sites at the points of strand exchange. Mutational analyses indicate that not all of the arm-type sites are required for integration, and that the P3 site and the rightmost pair of sites (P6 and P7) are dispensable for Integration. We show that a 252 bp segment of attP DNA is sufficient for efficient integrative recombination and that int can be provided in trans for simple and efficient transformation of the mycobacteria. |
| Keywords: | controlled study; sequence deletion; nonhuman; chromosome; dna recombination; cloning, molecular; molecular sequence data; recombination, genetic; escherichia coli; base sequence; binding sites; dna mutational analysis; mycobacterium; chromosomes, bacterial; mycobacterium smegmatis; integrase; integrases; consensus sequence; virus cell interaction; genome, viral; dna footprinting; mycobacteria; site-specific recombination; integration; bacteriophage; bacteriophages; virus integration; deoxyribonuclease i; priority journal; article; lysogenization; bacterium transformation; attachment site |
| Journal Title: | Journal of Molecular Biology |
| Volume: | 266 |
| Issue: | 1 |
| ISSN: | 0022-2836 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 1997-02-14 |
| Start Page: | 76 |
| End Page: | 92 |
| Language: | English |
| DOI: | 10.1006/jmbi.1996.0774 |
| PUBMED: | 9054972 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
