Expression of the fructose transporter GLUT5 in human breast cancer Journal Article
| Authors: | Zamora-León, S. P.; Golde, D. W.; Concha, I. I.; Rivas, C. I.; Delgado-López, F.; Baselga, J.; Nualart, F.; Vera, J. C. |
| Article Title: | Expression of the fructose transporter GLUT5 in human breast cancer |
| Abstract: | The primary metabolic characteristic of malignant cells is an increased uptake of glucose and its anaerobic metabolism. We studied the expression and function of the glucose transporters in human breast cancer cell lines and analyzed their expression in normal and neoplastic primary human breast tissue. Hexose uptake assays and immunoblotting experiments revealed that the breast carcinoma cell lines MCF-7 and MDA-468 express the glucose transporters GLUT1 and GLUT2, isoforms expressed in both normal and neoplastic breast tissue. We also found that the breast cancer cell lines transport fructose and express the fructose transporter GLUT5. Immunolocalization studies revealed that GLUT5 is highly expressed in vivo in human breast cancer but is absent in normal human breast tissue. These findings indicate that human breast cancer cells have a specialized capacity to transport fructose, a metabolic substrate believed to he used by few human tissues. Identification of a high-affinity fructose transporter on human breast cancer cells opens opportunities to develop novel strategies for early diagnosis and treatment of breast cancer. |
| Keywords: | human tissue; tumor localization; breast cancer; gene expression; breast; tumor cells, cultured; breast neoplasms; cancer cell; epithelium; pancreas islet beta cell; glucose transport; glucose transporter type 1; tissue specificity; glucose metabolism; immunologic techniques; deoxyglucose; monosaccharide transport proteins; fructose; humans; human; priority journal; article; anaerobic metabolism; fructose metabolism; glucose transporter type 2; glucose transporter type 5 |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 93 |
| Issue: | 5 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1996-03-05 |
| Start Page: | 1847 |
| End Page: | 1852 |
| Language: | English |
| DOI: | 10.1073/pnas.93.5.1847 |
| PUBMED: | 8700847 |
| PROVIDER: | scopus |
| PMCID: | PMC39870 |
| DOI/URL: | |
| Notes: | Erratum issued, see: Proc Natl Acad Sci U S A. 1996 Dec 24; 93(26):15522 -- Pilar S. Zamora-León's First name and initial are reversed on the original publication -- Article -- Export Date: 22 November 2017 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work