Hexose transporter expression and function in mammalian spermatozoa: Cellular localization and transport of hexoses and vitamin C Journal Article

Authors: Angulo, C.; Rauch, M. C.; Droppelmann, A.; Reyes, A. M.; Slebe, J. C.; Delgado-López, F.; Guaiquil, V. H.; Vera, J. C.; Concha, I. I.
Article Title: Hexose transporter expression and function in mammalian spermatozoa: Cellular localization and transport of hexoses and vitamin C
Abstract: We analyzed the expression of hexose transporters in human testis and in human, rat, and bull spermatozoa and studied the uptake of hexoses and vitamin C in ball spermatozoa. Immunocytochemical and reverse transcription- polymerase chain reaction analyses demonstrated that adult human testis expressed the hexose transporters GLUT1, GLUT2, GLUT3, GLUT4, and GLUT5. Immunoblotting experiments demonstrated the presence of proteins of about 50- 70 kD reactive with anti-GLUT1, GLUT2, GLUT3, and GLUT5 in membranes prepared from human spermatozoa, but no proteins reactive with GLUT4 antibodies were detected. Immunolocalization experiments confirmed the presence of GLUT1, GLUT2, GLUT3, GLUT5, and low levels of GLUT4 in human, rat, and bull spermatozoa. Each transporter isoform showed a typical subcellular localization in the head and the sperm tail. In the tail, GLUT3 and GLUT5 were present at the level of the middle piece in the three species examined, GLUT1 was present in the principal piece, and the localization of GLUT2 differed according of the species examined. Bull spermatozoa transported deoxyglucose, fructose, and the oxidized form of vitamin C, dehydroascorbic acid. Transport of deoxyglucose and dehydroascorbic acid was inhibited by cytochalasin B, indicating the direct participation of facilitative hexose transporters in the transport of both substrates by bull spermatozoa. Transport of fructose was not affected by cytochalasin B, which is consistent for an important role for GLUT5 in the transport of fructose in these cells. The data show that human, rat, and bull spermatozoa express several hexose transporter isoforms that allow for the efficient uptake of glucose, fructose, and dehydroascorbic acid by these cells.
Keywords: controlled study; protein expression; human cell; nonhuman; animal cell; mammalia; animals; animalia; immunocytochemistry; rat; cellular distribution; species difference; base sequence; cattle; ascorbic acid; dna primers; dehydroascorbic acid; spermatozoon; spermatozoa; semen analysis; sperm; cytochalasin b; deoxyglucose; hexoses; monosaccharide transport proteins; fructose; humans; human; male; priority journal; article; glucose transporters; hexose transport; 2-deoxy-d- glucose; hexose
Journal Title: Journal of Cellular Biochemistry
Volume: 71
Issue: 2
ISSN: 0730-2312
Publisher: John Wiley & Sons  
Date Published: 1998-11-01
Start Page: 189
End Page: 203
Language: English
DOI: 10.1002/(sici)1097-4644(19981101)71:2<189::aid-jcb5>3.0.co;2-r
PUBMED: 9779818
PROVIDER: scopus
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
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MSK Authors
  1. Juan C Vera
    59 Vera