| Abstract: |
Autonomously replicating sequence (ARS) elements of the fission yeast Schizosaccharomyces pombe contain multiple imperfect copies of the consensus sequence reported by Maundrell et al. [Maundrell K., Hutchison, A. and Shall, S. (1988) EMBO J. 7, 2203-2209]. When cell free extracts of S. pombe were incubated with a dimer or tetramer of an oligonucleotide containing the ARS consensus sequence, several complexes were detected using a gel mobility- shift assay. The proteins forming these complexes also bind ars3002, which is the most active origin in the ura4 region of chromosome III of S. pombe. One protein, partly responsible for the binding activity observed with crude extracts, was purified to near homogeneity. It is a 60-kDa protein and was named ARS-binding protein 1 (Abp1). Abp1 preferentially hinds to multiple sites in ARS 3002 and to the DNA polymer poly[d(A·T)]. The cloning and sequence of the gene coding for Abp1 revealed that it encodes a protein of 59.8 kDa (522 amino acids). Abp1 has significant homology (25% identity, 50% similarity) to the N-terminal region (≃300) amino acids) of the human and mouse centromere DNA-binding protein CENP-B. Because centromeres of S. pombe contain a high density of ARS elements, Abp1 may play a role connecting DNA replication and chromosome segregation. |
| Keywords: |
dna-binding proteins; dna replication; animals; chromosomal proteins, non-histone; mice; protein binding; transcription factors; molecular cloning; cloning, molecular; amino acid sequence; molecular sequence data; sequence homology, amino acid; amino terminal sequence; sequence alignment; autoantigens; base sequence; binding protein; saccharomyces cerevisiae proteins; sequence homology; nucleotide repeat; schizosaccharomyces; schizosaccharomyces pombe proteins; schizosaccharomyces pombe; dna, fungal; fungal proteins; genes, fungal; dna-binding protein; humans; priority journal; article; cenp-b; centromere protein b
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