Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe Journal Article

   


Authors: Lee, J. K.; Huberman, J. A.; Hurwitz, J.
Article Title: Purification and characterization of a CENP-B homologue protein that binds to the centromeric K-type repeat DNA of Schizosaccharomyces pombe
Abstract: We have purified and characterized a novel 60-kDa protein that binds to centromeric K-type repeat DNA from Schizosaccharomyces pombe. This protein was initially purified by its ability to bind to the autonomously replicating sequence 3002 DNA. Cloning of the gene encoding this protein revealed that it possesses significant homology to the mammalian centromere DNA-binding protein CENP-B and S. pombe Abp1, and this gene was designated as cbh+ (CENP-B homologue)-Cbh protein specifically interacts in vitro with the K- type repeat DNA, which is essential for centromere function. The Cbh-binding consensus sequence was determined by DNase I footprinting assays as PyPuATATPyPuTA, featuring on inverted repeat of the first four nucleotides. Based on its binding activity to centromeric DNA and homology to centromere proteins, we suggest that this protein may be a functional homologue of the mammalian CENP-B in S. pombe.
Keywords: dna-binding proteins; binding affinity; mammalia; chromosomal proteins, non-histone; protein binding; enzyme activity; molecular cloning; cloning, molecular; amino acid sequence; molecular sequence data; sequence homology, amino acid; protein purification; chromatin; mammal; autoantigens; dna sequence; binding sites; protein secondary structure; dna binding; dna determination; repetitive sequences, nucleic acid; centromere; schizosaccharomyces; schizosaccharomyces pombe; deoxyribonuclease; dna, fungal; fungal proteins; dna footprinting; priority journal; article; centromere protein b
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 94
Issue: 16
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1997-08-05
Start Page: 8427
End Page: 8432
Language: English
DOI: 10.1073/pnas.94.16.8427
PUBMED: 9237993
PROVIDER: scopus
PMCID: PMC22944
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030873317&doi=10.1073%2fpnas.94.16.8427&partnerID=40&md5=cf16501501d82c222aba7bb34361fdbd
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus
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MSK Authors
  1. Joon-Kyu Lee
    9 Lee
  2. Jerard Hurwitz
    206 Hurwitz
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