A family of Shc related proteins with conserved PTB, CH1 and SH2 regions Journal Article
| Authors: | Pelicci, G.; Dente, L.; De Giuseppe, A.; Verducci-Galletti, B.; Giuli, S.; Mele, S.; Vetriani, C.; Giorgio, M.; Pandolfi, P. P.; Cesareni, G.; Pelicci, P. G. |
| Article Title: | A family of Shc related proteins with conserved PTB, CH1 and SH2 regions |
| Abstract: | Shc proteins are targets of activated tyrosine kinases and have been implicated in the transmission of activation signals to Ras. Upon phosphorylation. Shc proteins form stable complexes with cellular tyrosine-phosphorylated proteins and with the Grb2 adaptor protein. Two Shc isoforms of 52 and 46 kDa have been characterized. They share a C-terminal SH2 domain, a proline- and glycine-rich region (collagen homologous region 1; CH1) and a N-terminal phospho-tyrosine binding domain (PTB). We report here the initial characterization of two Shc related human cDNAs: ShcB and ShcC. The ShcB and ShcC cDNAs code for proteins that are highly similar and share the same modular organization as Shc. PTB and SH2 domains of ShcB and ShcC have similar binding specificities in vitro and bind to activated EGFR in a phosphotyrosine-dependent manner. Based on these findings we propose to rename Shc as ShcA. Anti-ShcB and anti-ShcC antibodies recognize specific polypeptides of 52, 47 kDa (ShcB) and 54 kDa (ShcC) in mammalian cells. Since these two genes are predominantly expressed in specific brain tissues, these Shc family members may be involved in cell type-specific signaling, in the nervous system. |
| Keywords: | signal transduction; controlled study; protein phosphorylation; human cell; dna-binding proteins; nonhuman; proteins; protein analysis; animal cell; mouse; mammalia; animal tissue; carboxy terminal sequence; epidermal growth factor receptor; protein binding; receptor, epidermal growth factor; tumor cells, cultured; enzyme substrate; protein tyrosine kinase; animalia; cloning, molecular; rna-binding proteins; amino acid sequence; conserved sequence; molecular sequence data; amino terminal sequence; rna, messenger; nucleotide sequence; antigen recognition; adaptor proteins, signal transducing; base sequence; binding site; protein family; molecular weight; brain protein; isoprotein; phosphoprotein; complementary dna; dna, complementary; adaptor proteins, vesicular transport; phosphotyrosine; brain tissue; chitinase; plant proteins; humans; human; priority journal; article; src homology domains; modular organization; shc family; shc related proteins; polypyrimidine tract-binding protein |
| Journal Title: | Oncogene |
| Volume: | 13 |
| Issue: | 3 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1996-08-01 |
| Start Page: | 633 |
| End Page: | 641 |
| Language: | English |
| PUBMED: | 8760305 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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