Immortalization of neuroendocrine pinealocytes from transgenic mice by targeted tumorigenesis using the tryptophan hydroxylase promoter Journal Article
| Authors: | Son, J. H.; Chung, J. H.; Huh, S. O.; Park, D. H.; Peng, C.; Rosenblum, M. G.; Chung, Y. I.; Joh, T. H. |
| Article Title: | Immortalization of neuroendocrine pinealocytes from transgenic mice by targeted tumorigenesis using the tryptophan hydroxylase promoter |
| Abstract: | Tryptophan hydroxylase (TPH) is the first enzyme in both serotonin and melatonin biosynthesis in neuroendocrine cells of the pineal gland. The lack of immortalized neuroendocrine pineal cell lines has been a major obstacle to the study of the tissue-specific and circadian regulation of TPH gene expression in the pineal gland. Previously, we demonstrated that a 6.1 kb 5' upstream region of the mouse TPH gene directs the restricted expression of a lacZ reporter gene to the pineal gland and the raphe nuclei of transgenic mice. Therefore, to develop TPH-expressing pineal cell lines we first established transgenic mice carrying a construct consisting of 6.1 kb of 5' flanking region fused to the SV40 T-antigen. These animals developed highly invasive pineal tumors and died at 12-15 weeks of age. The pineal tumors obtained from the transgenic mice were utilized to establish the immortalized pinealocyte-derived cell lines. These cells express two marker enzymes, TPH and serotonin N-acetyltransferase (NAT). In pineal gland TPH and NAT expressions have been known to be regulated during circadian cycle. The two established cell lines therefore promise to be a valuable in vitro model system for the study of the rhythmic nature of the pineal function at molecular level in mammal. |
| Keywords: | controlled study; nonhuman; mouse; mammalia; animal tissue; gene expression; animal experiment; carcinogenesis; transgenic mouse; animalia; mus musculus; biosynthesis; reporter gene; simian virus 40; dna flanking region; virus t antigen; pineal body tumor; neurosecretory cell; pineal body; cell immortalization; circadian rhythm; simiae; raphe nucleus; simian virus; priority journal; article; tryptophan hydroxylase; serotonin n-acetyltransferase; transgenic pineal tumor; tryptophan hydroxylase promoter; serotonin n acetyltransferase; pinealocyte |
| Journal Title: | Molecular Brain Research |
| Volume: | 37 |
| Issue: | 1-2 |
| ISSN: | 0169-328X |
| Publisher: | Elsevier B.V. |
| Date Published: | 1996-04-01 |
| Start Page: | 32 |
| End Page: | 40 |
| Language: | English |
| DOI: | 10.1016/0169-328x(95)00271-s |
| PUBMED: | 8738133 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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