Detection of c-erbB-2 and other tumor markers on bladder tumor specimens: Marked differences after formalin or Bouin's fixation Journal Article

  


Authors: Fradet, Y.; Lafleur, L.; Tetu, B.; Cordon-Cardo, C.; Herz, F.; Mayall, B. H.; NIH Marker Network for Bladder Cancer
Article Title: Detection of c-erbB-2 and other tumor markers on bladder tumor specimens: Marked differences after formalin or Bouin's fixation
Abstract: In the course of a multi-center retrospective study on the prognostic value of bladder tumor markers, it became apparent that tumor samples from one of the Centers showed reactivity patterns outside the normal range of variability. The antibodies investigated were a rabbit antiserum to c-erbB- 2, the monoclonal antibody P12 to LewisX and the monoclonal antibodies (mAb) M344 and 19A211 to bladder tumor-associated antigens. The tumor specimens (total=171) were from comparable patient populations from each Center. The only difference was in the fixation of the tumors, done with formalin in three Centers (12 tumors) and with Bouin's solution (50 tumors) in one Center. Analysis of the results showed significant differences in the rate of positivity between comparable tumors. Positivity of Mab 19A211 with Bouin's tumors was 30% of that with formalin-fixed tumors, while reactivity with the antiserum to c-erbB-2 was four fold more frequent in Bouin's-fixed tumors. The expression of these two antigens is clearly different from that expected from preliminary results and from other published studies. Thus, tissue fixation with Bouin's solution can generate artificially high or low results with some antibodies. This may have important implications in the study of tumor markers by immunohistochemistry.
Keywords: immunohistochemistry; cancer survival; human tissue; cancer diagnosis; tumor marker; bladder tumor; monoclonal antibody; tissue fixation; c-erbb-2; her-2/neu; human; priority journal; article; human bladder neoplasm
Journal Title: Journal of Experimental & Clinical Cancer Research
Volume: 15
Issue: 2
ISSN: 1756-9966
Publisher: Biomed Central Ltd  
Date Published: 1996-06-01
Start Page: 99
End Page: 102
Language: English
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029793584&partnerID=40&md5=c1de1c75125090504ff3b8eac79bae33
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus