Decreased insulin-like growth factor I receptor expression and function in immortalized African Pygmy T cells Journal Article
| Authors: | Hattori, Y.; Vera, J. C.; Rivas, C. I.; Bersch, N.; Bailey, R. C.; Geffner, M. E.; Golde, D. W. |
| Article Title: | Decreased insulin-like growth factor I receptor expression and function in immortalized African Pygmy T cells |
| Abstract: | Efe Pygmies of northeast Zaire have the shortest mean adult stature of any population on earth. Although various alterations in the GH/insulin-like growth factor I (IGF-I) axis have been suggested, the basis for short stature in the Pygmy is unknown. We previously described IGF-I unresponsiveness in a T lymphoblast cell line derived from an Efe Pygmy, and studies in five additional lines have confirmed severe IGF-I resistance in these cells. We have now performed experiments to determine the molecular basis for the IGF- I resistance in these cells. We found markedly decreased cell surface expression of IGF-I receptors with normal ligand binding affinity. The Pygmy IGF-I receptors were not autophosphorylated and did not transmit a signal in response to physiological concentrations of IGF-I. There was a substantially decreased level of IGF-I receptor messenger ribonucleic acid in the Pygmy cells with a normal messenger ribonucleic acid half-life. The nucleotide sequence of the full-length IGF receptor complementary DNA in Pygmy 1 showed no significant variation. These results indicate decreased IGF-I receptor gene transcription and IGF-I receptor signaling as the primary variation in the Pygmy cell lines. The findings point to the IGF-I receptor as the locus governing short stature in the African Pygmy and suggest that human stature may be genetically controlled by expression of the IGF-I receptor. |
| Keywords: | signal transduction; adult; controlled study; human cell; t-lymphocytes; gene expression; autophosphorylation; short stature; somatomedin c receptor; molecular sequence data; messenger rna; rna, messenger; nucleotide sequence; cell line, transformed; base sequence; somatomedin c; insulin-like growth factor i; ligand binding; human experiment; dna, complementary; continental population groups; intracellular membranes; cell immortalization; molecular probes; oceanic ancestry group; humans; human; male; female; priority journal; article; t lymphoblast; democratic republic of the congo; receptors, somatomedin |
| Journal Title: | Journal of Clinical Endocrinology and Metabolism |
| Volume: | 81 |
| Issue: | 6 |
| ISSN: | 0021-972X |
| Publisher: | Oxford University Press |
| Date Published: | 1996-06-01 |
| Start Page: | 2257 |
| End Page: | 2263 |
| Language: | English |
| DOI: | 10.1210/jc.81.6.2257 |
| PUBMED: | 8964861 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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