A v-SNARE implicated in intra-Golgi transport Journal Article
| Authors: | Nagahama, M.; Orci, L.; Ravazzola, M.; Amherdt, M.; Lacomis, L.; Tempst, P.; Rothman, J. E.; Söllner, T. H. |
| Article Title: | A v-SNARE implicated in intra-Golgi transport |
| Abstract: | We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, a Golgi SNARE of 28 kD, is efficiently packaged into Golgi- derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind α-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP- coated vesicles, but do accumulate docked uncoated vesicles. |
| Keywords: | controlled study; nonhuman; animal cell; animals; nerve tissue proteins; membrane proteins; animalia; amino acid sequence; molecular sequence data; hybrid protein; immunoglobulin g; brain; carrier proteins; cytoplasm; base sequence; antibody specificity; cell fractionation; dna sequence; cattle; membrane transport; biological transport; cytosol; complementary dna; golgi complex; cho cell; cho cells; cricetinae; membrane vesicle; vesicular transport proteins; binding, competitive; intracellular membranes; coated vesicles; membrane receptor; golgi apparatus; snare proteins; bos taurus; guanosine 5' o (3 thiotriphosphate); microscopy, immunoelectron; priority journal; article; soluble n-ethylmaleimide-sensitive factor attachment proteins; n ethylmaleimide |
| Journal Title: | Journal of Cell Biology |
| Volume: | 133 |
| Issue: | 3 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1996-05-01 |
| Start Page: | 507 |
| End Page: | 516 |
| Language: | English |
| DOI: | 10.1083/jcb.133.3.507 |
| PUBMED: | 8636227 |
| PROVIDER: | scopus |
| PMCID: | PMC2120813 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work