Interaction of transforming growth factor-β receptor I with farnesyl-protein transferase-α in yeast and mammalian cells Journal Article
| Authors: | Ventura, F.; Liu, F.; Doody, J.; Massagué, J. |
| Article Title: | Interaction of transforming growth factor-β receptor I with farnesyl-protein transferase-α in yeast and mammalian cells |
| Abstract: | Transforming growth factor β (TGF-β) signals through two transmembrane serine/threonine kinases, known as TβR-I and TβR-II. Several lines of evidence suggest that TβR-II acts as a primary receptor, binding TGF-β and phosphorylating TβR-I whose kinase activity then propagates the signal to unknown substrates. We report an interaction between TβR-I and the farnesylprotein transferase-α subunit (FT-α) both in a yeast two-hybrid system and in mammalian cells. These findings raise the possibility that TGF-β might regulate cellular functions by altering the ability of FT-α to catalyze isoprenylation of targets such as G proteins, lamins, or cytoskeletal components. However, we provide evidence that TGF-β action does not alter the overall protein isoprenyl transferase activity in Mv1Lu mink lung epithelial cells. In fact, the β subunits of farnesyl transferase and geranylgeranyl transferase, which are necessary for the activity of FT-α, prevent the association of FT-α with TβR-I. Furthermore, farnesyl transferase activity is shown to be dispensable for TGF-β signaling of growth inhibitory and transcriptional responses in these cells. These results suggest that the interaction between TβR-I and FT-α does not affect the known functions of these two proteins. |
| Keywords: | human cell; nonhuman; protein analysis; mammalia; animals; gene expression; transforming growth factor beta; protein protein interaction; cell line; genetic transcription; enzyme activity; hela cells; transfection; cercopithecus aethiops; transcription regulation; amino acid sequence; molecular sequence data; kinetics; saccharomyces cerevisiae; enzyme inhibitors; protein-serine-threonine kinases; recombinant proteins; transforming growth factor beta receptor; receptors, transforming growth factor beta; binding sites; mammals; macromolecular substances; transferases; isoenzyme; receptor subtype; hybrid cell; protein isoprenylation; activin receptors, type i; humans; human; priority journal; article; farnesyl trans transferase; alkyl and aryl transferases; protein farnesyltransferase; mustela vison |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 271 |
| Issue: | 24 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1996-06-14 |
| Start Page: | 13931 |
| End Page: | 13934 |
| Language: | English |
| PUBMED: | 8663177 |
| PROVIDER: | scopus |
| DOI: | 10.1074/jbc.271.24.13931 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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