Ligand-induced recruitment and phosphorylation of reduced TGF-β type I receptor Journal Article
| Authors: | Vivien, D.; Wrana, J. L. |
| Article Title: | Ligand-induced recruitment and phosphorylation of reduced TGF-β type I receptor |
| Abstract: | Receptors I (TβR-I) and II (TβR-II) of transforming growth factor-β (TGF-β) are components of a heteromeric complex in which receptor I requires receptor II to bind ligand, and receptor II requires receptor I to signal. We show that pretreatment of cells with low concentrations of dithiothreitol, which is known to disrupt ligand binding to TβR-I, does not prevent interaction and complex formation between TβR-II and TβR-I. Nevertheless, our results demonstrate that ligand interaction with TβR-I is able to induce high-affinity convertion to the complex formed. We also demonstrate that transphosphorylation of TβR-I through bound TβR-II can occur independently of ligand binding to TβR-I. © 1995 Academic Press, Inc. |
| Keywords: | protein phosphorylation; nonhuman; binding affinity; animal cell; transforming growth factor beta; animalia; transforming growth factor beta receptor; ligand binding; mink; receptor subtype; priority journal; article; mustela vison |
| Journal Title: | Experimental Cell Research |
| Volume: | 221 |
| Issue: | 1 |
| ISSN: | 0014-4827 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1995-11-01 |
| Start Page: | 60 |
| End Page: | 65 |
| Language: | English |
| DOI: | 10.1006/excr.1995.1352 |
| PUBMED: | 7589256 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
