Purification, microsequencing, and immunolocalization of p36, a new interferon-α-induced protein that is associated with human lupus inclusions Journal Article
| Authors: | Rich, S. A.; Bose, M.; Tempst, P.; Rudofsky, U. H. |
| Article Title: | Purification, microsequencing, and immunolocalization of p36, a new interferon-α-induced protein that is associated with human lupus inclusions |
| Abstract: | The trace interferon-α-induced protein, p36, was induced in Raji cells in association with lupus inclusions. It was solubilized in a nonionic detergent buffer, enriched by differential centrifugation and by preparative isoelectric focusing, and purified to homogeneity on two-dimensional protein gels. Failure to obtain N-terminal amino acid sequence, however, suggested a blocked α-amino group. Sequences of six tryptic peptides, 13-19 amino acids in length, were obtained after digestion, microbore-high performance liquid chromotography purification, and chemical sequence analysis. None of the six sequences, which represented approximately 25% of the entire protein, shared any meaningful homologies with entries in protein sequence repositories. Raji-cell p36 was shown in Western blots with antipeptide antibodies to be induced at least 400-fold and by immunofluorescence microscopy to co-localize with the endoplasmic reticulum resident protein, protein disulfide isomerase. These results show that p36 is a new interferon-α-induced protein that localizes in the endoplasmic reticulum, the cell region in which the lupus inclusions form, and that p36 is probably physically associated with the lupus inclusions. |
| Keywords: | human cell; sequence analysis; alpha interferon; protein localization; microscopy, electron; cell protein; tumor cells, cultured; b-lymphocytes; endoplasmic reticulum; amino acid sequence; molecular sequence data; protein purification; cellular distribution; high performance liquid chromatography; chromatography, high pressure liquid; molecular weight; protein determination; interferon-alpha; annexin a2; cell inclusion; cytoplasmic granules; lupus erythematosus; lupus; humans; human; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 271 |
| Issue: | 2 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1996-01-12 |
| Start Page: | 1118 |
| End Page: | 1126 |
| Language: | English |
| DOI: | 10.1074/jbc.271.2.1118 |
| PUBMED: | 8557639 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
