Cytokine gene modification of bladder cancer cells for the establishment of bladder cancer vaccine Journal Article
| Authors: | Saito, S.; Tazaki, H.; Heston, W. D. W.; Fair, W. R. |
| Article Title: | Cytokine gene modification of bladder cancer cells for the establishment of bladder cancer vaccine |
| Abstract: | A bladder tumor vaccine in mouse models was established by introducing interleukin-2 (IL-2) gene into mouse bladder cancer cells (MBT-2). Mice injected with non-modified MBT-2 cells did not reject subsequent challenge of MBT-2 cells. Mice injected with IL-2-gene-modified MBT-2 cells (MBT-2/IL-2) rejected subsequent challenge of unmodified MBT-2 cells but not 38C13 cells, which have the same genetic background but different antigenicity. According to these results, an injection of MBT-2/II.-2 can establish specific immunity against parental MBT-2 cells in mice, which is also demonstrated by cytotoxic T lymphocytes assay. Vaccinations with MBT-2/IL-2 cured 60% of bladder cancer which had been pre-established in mice. These results suggest that tumor cells genetically modified with IL-2 gene can act as a tumor vaccine which is expected to be a new method for immunotherapy of cancers. © IUA/CLJ. |
| Keywords: | controlled study; genetics; nonhuman; conference paper; methodology; t lymphocyte; t-lymphocytes; animal cell; mouse; animal; cytology; animals; mice; interleukin 2; cancer immunotherapy; animal experiment; animal model; cancer cell culture; tumor cell culture; tumor cells, cultured; bladder cancer; bladder tumor; urinary bladder neoplasms; disease model; immunology; genetic transfection; immunotherapy; cancer vaccine; cancer vaccines; immunogenicity; cytotoxic t lymphocyte; gene therapy; adoptive transfer; neoplasm transplantation; disease models, animal; tumor vaccine; carcinoma, transitional cell; transitional cell carcinoma; adoptive immunotherapy; immunotherapy, adoptive; interleukin-2; cancer transplantation; mice, inbred c3h; c3h mouse; genetic therapy; female; priority journal; article; il2 protein, human |
| Journal Title: | International Journal of Urology |
| Volume: | 3 |
| Issue: | Suppl. 1 |
| ISSN: | 0919-8172 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1996-01-01 |
| Start Page: | S35 |
| End Page: | S39 |
| Language: | English |
| DOI: | 10.1111/j.1442-2042.1996.tb00080.x |
| PUBMED: | 24304018 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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