Genetic analysis of Rap1p/Sir3p interactions in telomeric and HML silencing in Saccharomyces cerevisiae Journal Article


Authors: Liu, C.; Lustig, A. J.
Article Title: Genetic analysis of Rap1p/Sir3p interactions in telomeric and HML silencing in Saccharomyces cerevisiae
Abstract: We have identified three SIR3 suppressors of the telomeric silencing defects conferred by missense mutations within the Rap1p C-terminal tail domain (aa800-827). Each SIR3 suppressor was also capable of suppressing a ra1p allele (rap1-21), which deletes the 28 aa C-terminal allele domaine, but none of suppressors restored telomeric silencing to a 165 amino acid truncation allele. These data suggest a Ra1p site for Sir3p association between the two truncation points (aa 664-799). In SIR3 suppressor strains lacking the Rap1p C-terminal tail domain, the presence of a second intragenic mutation within the rap1s domain (aa 727-747), enhanced silencing 30-300 fold. These data suggest a competition between Sir3p and factors that interfere with silencing for association in the rap1 domain, rap1-21 strains containing both wild-type Sir3p and either of the Sir3 suppressor proteins displayed a 400-4000-fold increase in telomeric silencing over rap1-21 strains carrying either Sir3p suppressor in the absence of wild-type Sir3p. We propose that this telomere-specific synergism is medicated in part through stabilization of Rap1p/Sir3p telomeric complexes by Sir3p-Sir3p interactions.
Keywords: missense mutation; nonhuman; genetic analysis; telomere; carboxy terminal sequence; gtp-binding proteins; genotype; alleles; transcription, genetic; transcription factors; transcription regulation; saccharomyces cerevisiae; histone; peptides; binding site; binding sites; trans-activators; chromosomes, fungal; fungal proteins; genes, fungal; restriction mapping; suppression, genetic; priority journal; article; silent information regulator proteins, saccharomyces cerevisiae; rap gtp-binding proteins; peptide biosynthesis
Journal Title: Genetics
Volume: 143
Issue: 1
ISSN: 0016-6731
Publisher: Genetics Society of America  
Date Published: 1996-05-01
Start Page: 81
End Page: 93
Language: English
PUBMED: 8722764
PROVIDER: scopus
PMCID: PMC1207297
DOI/URL:
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
Citation Impact
MSK Authors
  1. Arthur J. Lustig
    10 Lustig