Detection of SPO11-oligonucleotide complexes from mouse testes Journal Article

   


Authors: Pan, J.; Keeney, S.
Article Title: Detection of SPO11-oligonucleotide complexes from mouse testes
Abstract: The SPO11 protein generates programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination. Endonucleolytic cleavage 3' to the DSB sites releases SPO11 from DNA, leaving SPO11 covalently associated with an oligonucleotide. This chapter describes detection of the release product, SPO11-oligonucleotide complexes, from mouse testis lysates. The method for determining the size of SPO11-associated oligonucleotides is also provided.
Keywords: review; methodology; mouse; animal; metabolism; animals; mice; protein binding; chemistry; immunoprecipitation; dna breaks, double-stranded; double stranded dna break; oligonucleotide; testis; spo11 protein; esterase; macromolecule; esterases; macromolecular substances; oligonucleotides
Journal Title: Methods in Molecular Biology
Volume: 557
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2009-01-01
Start Page: 197
End Page: 207
Language: English
DOI: 10.1007/978-1-59745-527-5_13
PUBMED: 19799184
PROVIDER: scopus
PMCID: PMC3157917
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-70350780260&partnerID=40&md5=6082f136c30e0885c29b2bc656bb0714
Notes: Chapter 13 in "Meiosis: Volume 1, Molecular and Genetic Methods" (ISBN: 978-1-934115-66-4) --- Export Date: 30 November 2010 - Source: Scopus
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MSK Authors
  1. Scott N Keeney
    138 Keeney
  2. Jing Pan
    7 Pan
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