Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor Journal Article
| Authors: | Pan, Y. X.; Xu, J.; Pasternak, G. W. |
| Article Title: | Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor |
| Abstract: | By using a reverse transcription-PCR approach we have cloned a peptide precursor from mouse brain which contains the sequence of orphanin FQ/nociceptin. The mouse sequence of orphanin FQ/nociceptin is identical at the amino acid level with that isolated from rat and porcine brain. Northern analysis of the mRNA encoding the precursor reveals a single band of approx. 1 kb, with the highest levels in the brain and much lower levels in kidney and spleen. Southern analysis is consistent with a single gene. The precursor peptide from mouse contains two other putative peptides. Upstream from the orphanin FQ/nociceptin is a 41-amino-acid peptide which is almost identical, except for a six-amino-acid insertion, with the corresponding 35-amino-acid peptide predicted from the rat sequence. Interestingly, the mouse contains a triple AEPGAD repeat within this peptide that is not seen in the rat sequence. Downstream from the orphanin FQ/nociceptin sequence is another 17-amino-acid peptide which is identical with that found in the rat. |
| Keywords: | nonhuman; polymerase chain reaction; mouse; animals; mice; reverse transcription polymerase chain reaction; gene expression; spleen; molecular cloning; cloning, molecular; kidney; amino acid sequence; molecular sequence data; brain; messenger rna; rna, messenger; base sequence; rats; swine; complementary dna; dna, complementary; northern blotting; protein precursors; opioid peptides; southern blotting; kappa opiate receptor; receptors, opioid; priority journal; article |
| Journal Title: | Biochemical Journal |
| Volume: | 315 |
| Issue: | 1 |
| ISSN: | 0264-6021 |
| Publisher: | Portland Press Ltd |
| Date Published: | 1996-04-01 |
| Start Page: | 11 |
| End Page: | 13 |
| Language: | English |
| PUBMED: | 8670093 |
| PROVIDER: | scopus |
| PMCID: | PMC1217157 |
| DOI: | 10.1042/bj3150011 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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