MRD detection in multiple myeloma: Comparison between MSKCC 10-color single-tube and EuroFlow 8-color 2-tube methods Journal Article
| Authors: | Roshal, M.; Flores-Montero, J. A.; Gao, Q.; Koeber, M.; Wardrope, J.; Durie, B. G. M.; Dogan, A.; Orfao, A.; Landgren, O. |
| Article Title: | MRD detection in multiple myeloma: Comparison between MSKCC 10-color single-tube and EuroFlow 8-color 2-tube methods |
| Abstract: | In patients with multiple myeloma, obtaining posttreatment minimal residual disease (MRD) negativity is associated with longer progression-free survival and overall survival. Here, we compared the diagnostic performance of a single 10-color tube with that of a EuroFlow 8-color 2-tube panel forMRDtesting. Bone marrow samples from 41 multiple myeloma patients were tested in parallel using the 2 approaches. Compared with the sum of the cells from the EuroFlow two 8-color tubes, the Memorial Sloan Kettering Cancer Center (MSKCC) single 10color tube had a slight reduction in total cell number with a mean ratio of 0.85 (range, 0.571.46; P < .05), likely attributable to permeabilization of the cells. Percent of plasma cells showed a high degree of concordance (r(2) = 0.97) as did normal plasma cells (r(2) = 0.96), consistent with no selective plasma cell loss. Importantly, concordant measurement of residual disease burden was seen with abnormal plasma cells (r(2) = 0.97). The overall concordance between the 2 tests was 98%. In 1 case, there was a discrepancy near the limit of detection of both tests in favor of the slightly greater theoretical sensitivity of the EuroFlow 8-color 2-tube panel (analytical sensitivity limit of MSKCC single 10-color tube: 6 cells in 1 million with at least 3 million cell acquisitions; EuroFlow 8-color 2-tube panel: 2 cells in 1 million with the recommended 10 million cell acquisitions). |
| Keywords: | minimal residual disease; myeloma; impact; plasma-cell; multiparameter flow-cytometry |
| Journal Title: | Blood Advances |
| Volume: | 1 |
| Issue: | 12 |
| ISSN: | 2473-9529 |
| Publisher: | American Society of Hematology |
| Date Published: | 2017-05-09 |
| Start Page: | 728 |
| End Page: | 732 |
| Language: | English |
| ACCESSION: | WOS:000400980900003 |
| DOI: | 10.1182/bloodadvances.2016003715 |
| PROVIDER: | wos |
| PMCID: | PMC5728052 |
| PUBMED: | 29296716 |
| Notes: | Article -- Source: Wos |
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