PCP and SAX-3/Robo pathways cooperate to regulate convergent extension-based nerve cord assembly in C. elegans Journal Article

   

Authors:	Shah, P. K.; Tanner, M. R.; Kovacevic, I.; Rankin, A.; Marshall, T. E.; Noblett, N.; Tran, N. N.; Roenspies, T.; Hung, J.; Chen, Z.; Slatculescu, C.; Perkins, T. J.; Bao, Z.; Colavita, A.
Article Title:	PCP and SAX-3/Robo pathways cooperate to regulate convergent extension-based nerve cord assembly in C. elegans
Abstract:	Formation and resolution of multicellular rosettes can drive convergent extension (CE) type cell rearrangements during tissue morphogenesis. Rosette dynamics are regulated by both planar cell polarity (PCP)-dependent and -independent pathways. Here we show that CE is involved in ventral nerve cord (VNC) assembly in Caenorhabditis elegans. We show that a VANG-1/Van Gogh and PRKL-1/Prickle containing PCP pathway and a Slit-independent SAX-3/Robo pathway cooperate to regulate, via rosette intermediaries, the intercalation of post-mitotic neuronal cell bodies during VNC formation. We show that VANG-1 and SAX-3 are localized to contracting edges and rosette foci and act to specify edge contraction during rosette formation and to mediate timely rosette resolution. Simultaneous loss of both pathways severely curtails CE resulting in a shortened, anteriorly displaced distribution of VNC neurons at hatching. Our results establish rosette-based CE as an evolutionarily conserved mechanism of nerve cord morphogenesis and reveal a role for SAX-3/Robo in this process. © 2017 Elsevier Inc.
Keywords:	signal transduction; controlled study; unclassified drug; nonhuman; protein localization; embryo; cell protein; cellular distribution; cell polarity; caenorhabditis elegans; planar cell polarity; cell contact; intercalation complex; roundabout receptor; convergent extension; priority journal; article; convergent evolution; ventral root; cell body; convergent-extension; sax-3/robo; vang-1/van gogh; sax 3 protein; nerve cord
Journal Title:	Developmental Cell
Volume:	41
Issue:	2
ISSN:	1534-5807
Publisher:	Cell Press
Date Published:	2017-04-24
Start Page:	195
End Page:	203.e3
Language:	English
DOI:	10.1016/j.devcel.2017.03.024
PROVIDER:	scopus
PUBMED:	28441532
PMCID:	PMC5469364
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018818669&doi=10.1016%2fj.devcel.2017.03.024&partnerID=40&md5=9442d8daaee4d10af6cc0259629f06fd
Notes:	Article -- Export Date: 2 June 2017 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/110407