Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide Journal Article

   


Authors: Alam, S. M.; Aussedat, B.; Vohra, Y.; Meyerhoff, R. R.; Cale, E. M.; Walkowicz, W. E.; Radakovich, N. A.; Anasti, K.; Armand, L.; Parks, R.; Sutherland, L.; Scearce, R.; Joyce, M. G.; Pancera, M.; Druz, A.; Georgiev, I. S.; Von Holle, T.; Eaton, A.; Fox, C.; Reed, S. G.; Louder, M.; Bailer, R. T.; Morris, L.; Abdool-Karim, S. S.; Cohen, M.; Liao, H. X.; Montefiori, D. C.; Park, P. K.; Fernández-Tejada, A.; Wiehe, K.; Santra, S.; Kepler, T. B.; Saunders, K. O.; Sodroski, J.; Kwong, P. D.; Mascola, J. R.; Bonsignori, M.; Moody, M. A.; Danishefsky, S.; Haynes, B. F.
Article Title: Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide
Abstract: A goal for an HIV-1 vaccine is to overcome virus variability by inducing broadly neutralizing antibodies (bnAbs). One key target of bnAbs is the glycan-polypeptide at the base of the envelope (Env) third variable loop (V3). We have designed and synthesized a homogeneous minimal immunogen with high-mannose glycans reflective of a native Env V3-glycan bnAb epitope (Man9-V3). V3-glycan bnAbs bound to Man9-V3 glycopeptide and native-like gp140 trimers with similar affinities. Fluorophore-labeled Man9-V3 glycopeptides bound to bnAb memory B cells and were able to be used to isolate a V3-glycan bnAb from an HIV-1-infected individual. In rhesus macaques, immunization with Man9-V3 induced V3-glycan-targeted antibodies. Thus, the Man9-V3 glycopeptide closely mimics an HIV-1 V3-glycan bnAb epitope and can be used to isolate V3-glycan bnAbs. 2017 © The Authors, some rights reserved.
Journal Title: Science Translational Medicine
Volume: 9
Issue: 381
ISSN: 1946-6234
Publisher: American Association for the Advancement of Science  
Date Published: 2017-03-15
Start Page: eaai7521
Language: English
DOI: 10.1126/scitranslmed.aai7521
PROVIDER: scopus
PUBMED: 28298421
PMCID: PMC5562351
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016422011&doi=10.1126%2fscitranslmed.aai7521&partnerID=40&md5=262be75d51125cea87e4076e476a575e
Notes: Article -- Export Date: 2 May 2017 -- Source: Scopus
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MSK Authors
  1. Peter Kinam Park
    10 Park
  2. Baptiste Aussedat
    17 Aussedat
  3. Samuel J Danishefsky
    539 Danishefsky
  4. Alberto Fernandez-Tejada
    17 Fernandez-Tejada
  5. Yusufbhai Usmanbhai Vohra
    6 Vohra
  6. William Walkowicz
    9 Walkowicz
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