A novel technique for generating and observing chemiluminescence in a biological setting Journal Article
| Authors: | Büchel, G. E.; Carney, B.; Tang, J.; Zeglis, B. M.; Eppinger, J.; Reiner, T. |
| Article Title: | A novel technique for generating and observing chemiluminescence in a biological setting |
| Abstract: | Intraoperative imaging techniques have the potential to make surgical interventions safer and more effective; for these reasons, such techniques are quickly moving into the operating room. Here, we present a new approach that utilizes a technique not yet explored for intraoperative imaging: chemiluminescent imaging. This method employs a ruthenium-based chemiluminescent reporter along with a custom-built nebulizing system to produce ex vivo or in vivo images with high signal-to-noise ratios. The ruthenium-based reporter produces light following exposure to an aqueous oxidizing solution and re-reduction within the surrounding tissue. This method has allowed us to detect reporter concentrations as low as 6.9 pmol/cm2. In this work, we present a visual guide to our proof-of-concept in vivo studies involving subdermal and intravenous injections in mice. The results suggest that this technology is a promising candidate for further preclinical research and might ultimately become a useful tool in the operating room. © 2017 Journal of Visualized Experiments. |
| Keywords: | lymph nodes; in vivo; bioengineering; cerium; intraoperative imaging; chemiluminescence; ruthenium; issue 121 |
| Journal Title: | Journal of Visualized Experiments |
| Volume: | 2017 |
| Issue: | 121 |
| ISSN: | 1940-087X |
| Publisher: | MYJoVE Corporation |
| Date Published: | 2017-03-01 |
| Start Page: | e54694 |
| Language: | English |
| DOI: | 10.3791/54694 |
| PROVIDER: | scopus |
| PUBMED: | 28362395 |
| PMCID: | PMC5407651 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 May 2017 -- Source: Scopus |
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