Identification of a novel suppressive vitamin D response sequence in the 5'-Flanking region of the murine Id1 gene Journal Article

  


Authors: Ezura, Y.; Tournay, O.; Nifuji, A.; Noda, M.
Article Title: Identification of a novel suppressive vitamin D response sequence in the 5'-Flanking region of the murine Id1 gene
Abstract: Vitamin D promotes differentiation of cells either by simply enhancing phenotypic gene expression and/or by suppressing expression of inhibitors of differentiation. Previously, we reported that expression of a gene encoding Id1, a negative type helix-loop-helix transcription factor, was transcriptionally suppressed by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) (1). To identify the sequence required for the negative regulation by 1,25(OH)2D3, a 1.5-kilobase 5'-flanking region of murine Id1 gene was examined by transiently transfecting luciferase reporter constructs into ROS17/2.8 osteoblastic cells. The transcriptional activity of this construct was repressed by 10-8 M 1,25(OH)2D3. Deletion analysis revealed that a 57-base pair (bp) upstream response sequence (URS) (-1146/-1090) was required for the suppression by 1,25(OH)2D3. This sequence conferred negative responsiveness to 1,25(OH)2D3 to a heterologous SV40 promoter. The 57-bp URS contained not only Egr-1 consensus sequence (2) but also four direct repeats of a heptamer sequence (C/A)CAGCCC. Electrophoresis mobility shift assay revealed that the 57-bp URS formed specific nuclear protein-DNA complexes, which were neither competed by previously known positive and negative vitamin D response elements nor supershifted by anti-vitamin D receptor antibody, suggesting the absence of vitamin D receptor in these complexes. These results indicate the involvement of the novel 57-bp sequence in the vitamin D suppression of Id1 gene transcription.
Keywords: controlled study; sequence deletion; nonhuman; animal cell; mouse; phenotype; animals; cells, cultured; nuclear protein; luciferase; transcription factor; cell differentiation; transcription, genetic; calcitriol; animalia; transcription factors; nuclear proteins; gene expression regulation; transcription regulation; molecular sequence data; vitamin d; murinae; base sequence; dna flanking region; rats; inhibitor of differentiation protein 1; vitamin d receptor; repressor proteins; electrophoretic mobility; osteoblast; dna protein complex; receptor antibody; suppressor gene; helix loop helix protein; promoter regions (genetics); helix-loop-helix motifs; priority journal; article; hormone responsive element
Journal Title: Journal of Biological Chemistry
Volume: 272
Issue: 47
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 1997-11-21
Start Page: 29865
End Page: 29872
Language: English
DOI: 10.1074/jbc.272.47.29865
PUBMED: 9368060
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030701537&doi=10.1074%2fjbc.272.47.29865&partnerID=40&md5=aabd23d485fc4019b459fdbcbf3238a6
Notes: Article -- Export Date: 17 March 2017 -- Source: Scopus
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