Comparison of MUC-1 mucin expression in epithelial and non-epithelial cancer cell lines and demonstration of a new short variant form (MUC-1/Z) Journal Article
| Authors: | Oosterkamp, H. M.; Scheiner, L.; Stefanova, M. C.; Lloyd, K. O.; Finstad, C. L. |
| Article Title: | Comparison of MUC-1 mucin expression in epithelial and non-epithelial cancer cell lines and demonstration of a new short variant form (MUC-1/Z) |
| Abstract: | Mucins, including MUC-1, are generally considered to be products of epithelial tissues and of their tumors. To examine the possible expression of MUC-1 in other cell types, a panel of human epithelial and non-epithelial tumor cell lines was studied by reverse transcriptase polymerase chain reaction (RT-PCR). Northern blot analysis, immunocytology and radio- immunoprecipitation. Using the highly sensitive RT-PCR method, products corresponding to the non-repetitive 5' and 3' MUC-1 sequences were detected in all the cell lines examined. Amplified products lacking the tandem repeat region of MUC-1, including a new short form (designated MUC-1/Z) different from the previously reported MUC-1/Y protein, were also detected in most cell lines tested. Northern blot analysis, using a probe to the variable number tandem repeat (VNTR) region, confirmed the presence of MUC-1 mRNA in the astrocytoma, melanoma and neuroblastoma cell lines studied. MUC-1 protein was detected by immunocytology in these cell lines using monoclonal antibody (MAb) 139H2. Immunoprecipitation analysis with [3H]-glucosamine-labeled cell lysates and MAb 139H2 or an antibody to the cytoplasmic domain, CT-1, detected MUC-1 protein in 2 epithelial cell lines, an astrocytoma cell line (SK-MG-4) but not in the melanoma and neuroblastoma cell lines studied. Northern blot analysis using a probe to the 3' end of MUC-1 mRNA, confirmed the presence of MUC-1 mucin and also identified short products corresponding to the size of the short variant forms. Protein products corresponding to the MUC-1/Y and MUC-1/Z variant forms were not observed using either [3H]- glucosamine-labeled OVCAR-3 cells or [3H]-amino acid-labeled MCF-7 cells and either CT-1 antibody or MAb 232AI, detecting an epitope to the C-terminal region. Thus, depending on the sensitivity of the assay used, varying amounts of MUC-1 mRNA and protein could be detected in non-epithelial tumor cell lines. Although the amounts of MUC-1 in these cell lines are much lower than in carcinomas, it is possible that MUC-1 mucin serves a similar function in non-epithelial as in epithelial cells. The possible role of MUC-1/Y and MUC- 1/Z variant forms in these cell lines is not understood. |
| Keywords: | controlled study; protein expression; human cell; polymerase chain reaction; neoplasms; ovarian neoplasms; cytology; melanoma; reverse transcription polymerase chain reaction; neoplasm proteins; colonic neoplasms; tumor cells, cultured; breast neoplasms; kidney neoplasms; monoclonal antibody; dna; molecular sequence data; neuroblastoma; messenger rna; rna, messenger; cancer cell; epithelium cell; base sequence; amino acid; dna sequence; epithelium; blotting, northern; astrocytoma; antibody; immunochemistry; northern blotting; mucin; mucins; tandem repeat; radioimmunoprecipitation; ca-15-3 antigen; glucosamine; humans; human; female; priority journal; article |
| Journal Title: | International Journal of Cancer |
| Volume: | 72 |
| Issue: | 1 |
| ISSN: | 0020-7136 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1997-07-03 |
| Start Page: | 87 |
| End Page: | 94 |
| Language: | English |
| DOI: | 10.1002/(sici)1097-0215(19970703)72:1<87::aid-ijc13>3.0.co;2-7 |
| PUBMED: | 9212228 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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